辛伐他汀对成骨细胞血管内皮生长因子的表达和颅骨内血管新生的影响  

EFFECTS OF SIMVASTATIN ON OSTEOBLAST VEGF EXPRESSION AND MICE CALVARIA ANGIONENESIS

作　　者：杨允[1] 张立平[2] 李晓光[1] 胡坚莉[2] 张佐伦[3] 张凯宁[1] 张虎[1] 宋淑香[1] 

机构地区：[1]山东大学医学院山东省千佛山医院骨科,济南250014 [2]山东大学医学院解剖教研室,济南250012 [3]山东省立医院骨科,济南250016

出　　处：《解剖学报》2007年第5期564-568,共5页Acta Anatomica Sinica

基　　金：山东大学青年教师科研启动基金

摘　　要：目的通过观察辛伐他汀对体外培养成骨细胞血管内皮生长因子(VEGF)的表达及对颅骨血管新生的影响,探讨其促进骨形成的作用机制。方法将离体培养颅骨成骨细胞与辛伐他汀作用24h后,提取RNA行RT-PCR,半定量测定细胞VEGF mRNA的表达,免疫细胞化学染色吸光度测定VEGF蛋白表达水平;小鼠头皮下局部注射辛伐他汀和空白溶剂25μl,连续5d,1个月后取出颅骨做病理检查。结果辛伐他汀可增加成骨细胞VEGF的mRNA和蛋白的表达。局部用药后可见小鼠颅骨增厚,骨质内部有新生血管出现。结论促进成骨细胞VEGF的表达及骨内血管新生,是他汀类药物促进骨形成的作用机制之一。Objective To explore the mechanism of osteogenesis from statins through observing the effects of simvastatin on the VEGF expression of calvaria osteoblasts and its local influence on the mice calvaria. Methods Rat calvaria osteoblasts were harvested and cultured. Cells were treated with simvastatin for 24 hours. RT-PCR and immunocytology were performed to detect the expression of VEGF. Simvastatin was injected subcutaneously over the calvaria of mice for 4 days, the calvaria were pathologically examined. Results VEGF expression in rat calvaria osteoblasts was promoted by simvastain at both transcriptional and translational levels. In vitro the calvaria thickness and angiogenesis were increased by local administration of simvastatin. Conclusion Simvastatin can promote osteoblasts VEGF expression in vitro and mice calvaria angiogenesis and in vivo, which plays an important role in stimulating osteogenesis.

关 键 词：辛伐他汀 成骨细胞 血管新生 血管内皮细胞生长因子 RT-PCR 小鼠 大鼠 

分 类 号：R322.7[医药卫生—人体解剖和组织胚胎学]