野菊花总黄酮诱导佐剂性关节炎大鼠滑膜细胞凋亡  

INDUCTION OF APOPTOSIS BY EXTRACT OF TOTAL FLAVONOIDS OF CHRYSANTHEMUM INDICUM ON ADJUVANT ARTHRITIS SYNOVIAL CELLS

作　　者：陈晓宇[1] 李俊[1] 解雪峰[1] 姜辉[1] 韩卉[2] 

机构地区：[1]安徽医科大学药学院 [2]安徽医科大学解剖学教研室,合肥230032

出　　处：《解剖学报》2007年第5期569-571,共3页Acta Anatomica Sinica

基　　金：国家重大基础研究前期研究专项基金(2002CCC-02900);安徽省高等学校青年教师科研基金资助(2006jq100zd)

摘　　要：目的研究野菊花有效成分总黄酮(TFC)对佐剂性关节炎(AA)大鼠滑膜细胞凋亡的影响,探讨其治疗作用的机理。方法SD大鼠右后足跖皮内注射0.1ml弗氏完全佐剂复制AA模型;24d后组织块培养法分离培养大鼠膝关节滑膜细胞,用MTT法检测TFC对滑膜细胞增殖的影响;Hoechst 33258荧光染色,观察滑膜细胞核是否出现凋亡小体;琼脂糖凝胶电泳观察DNA碎片。结果MTT法显示TFC抑制滑膜细胞增殖,并且呈剂量依赖性趋势,药物作用24h的IC50为112mg/L;TFC能诱导滑膜细胞凋亡,电镜观察到典型的凋亡细胞核,电泳呈现出阶梯状条带,Hoechst 33258染色观察到细胞核出现凋亡小体。结论TFC可能会通过抑制AA大鼠滑膜细胞的增生,促进滑膜细胞的凋亡而达到治疗类风湿性关节炎的作用。Objective To study the effect of the extract of total flavonoids of chrysanthemum indicum（TFC） on adjuvant arthritis synovial cells. Methods 0. 1ml of the complete Freund＇ s adjuvant was subcutaneously injected into the right hind feet pads of the SD rats. 24 days after immunity synovial ceils in knee joint were treated with TFC and inhibition of proliferation was measured with MTY assay. DNA fragmentations were analyzed with DNA gel electrophoresis. Fluorescence staining of Hoechst 33258 to observe apoptotic body. Results The IC50 of TFC on synovial cells was 112mg/L. DNA gel electrophoresis showed ladder-like strap. Apoptotic bodies were observed by Hoecbst 33258. Conclusion TFC can inhibit proliferation and induce apoptosis in synovial cells, and exerts therapeutical effect on rheumstoid arthritis.

关 键 词：野菊花总黄酮 佐剂性关节炎 滑膜细胞 细胞凋亡 MTT法 DNA琼脂糖凝胶电泳 大鼠 

分 类 号：R322.72[医药卫生—人体解剖和组织胚胎学] R329.2[医药卫生—基础医学]