大豆异黄酮对去势大鼠脂质过氧化及肝超微结构的影响  

THE EFFECT OF SOYBEAN ISOFLAVONE ON LIPID PEROXIDATION AND LIVER ULTRASTRUCTURE IN OVARIECTOMIZED RATS

作　　者：余清[1] 楼新法[2] 王文蔚[1] 李安乐[3] 刘存丽[4] 王一龙[3] 胡万里[5] 

机构地区：[1]温州医学院环境与公共卫生学院 [2]温州医学院解剖学教研室 [3]温州医学院实验动物中心 [4]温州医学院第一附属医院检验科,温州325005 [5]温州医学院电镜室,温州325035

出　　处：《解剖学报》2007年第5期601-605,共5页Acta Anatomica Sinica

基　　金：浙江省教育厅科研项目(20041049);温州市科技局科研基金(Y2003A047)资助项目

摘　　要：目的研究大豆异黄酮(soybean isoflavone,SI)对去势大鼠脂质过氧化及肝超微结构的影响。方法70只雌性SD大鼠按血清总胆固醇水平随机分为7组:高脂、雌激素,低、中、高剂量SI干预、假手术及正常对照组。前5组摘除双侧卵巢,恢复1周后开始干预,实验周期为12周,灌胃给药,每周称重1次,分别在去卵巢、给药4、8周、处死时抽取尾静脉血,实验结束后取肝脏标本,分别测定胆固醇(TC)、甘油三酯(TG)、血清高、低密度脂蛋白胆固醇(HDL-C、LDL-C)、脂蛋白(a)及抗氧化酶活性等。结果SI干预组LDL-C水平显著低于高脂组但仍高于正常对照组;干预可使脂蛋白(a)水平发生变化但对HDL-C影响不显著;高剂量SI能缓解因去卵巢造成的肝细胞超微结构破坏,维护其完整性,全视野下以高剂量干预组与正常组结构最为接近,高脂组镜像损坏明显。结论SI对去卵巢大鼠的脂蛋白(a)、脂质过氧化及肝超微结构存在影响,高剂量持续干预能降低去卵巢大鼠的LDL-C,并能维持肝超微结构。Objective To study the effects of soybean isoflavone （SI） on lipid peroxidation and liver ultrastructure in ovariectomized rats. Methods Seventy female Sprague-Dawley（SD） rats were randomly divided into 7 groups according to the levels of total cholesterol（TC） in serum： hyper-lipoid group, estrogen group, low-dose SI group, middle-dose SI group, high-dose SI group, sham group and normal control group. After bilateral ovaries were extirpated except sham and normal control groups for a week, the estrogen, different doses of SI or deionized water were fed with intragastric administration for 12 weeks. The rat body weight was weighted once per week and blood samples were collected at the time of ovariectomization, the 4th and 8th weeks after administeration at the time of being killed. The serum TC, triglyceride（TG）, high or low density lipoprotein-cholesterol（ HDL-C, LDL-C）, lipoprotein（a） and antioxygen enzyme were assayed. Results LDL-C levels in SI intervention groups were significantly lower than that in hyper-lipoid groups but higher than that in normal control group; the levels of lipoprotein（a） were changed; there was almost no effect on HDL-C in serum. SI could retain integrity and ultrastructure of hepatocyte; there was similar structure in high dose SI group and normal control group. An obvious damage was detected in hyper-lipoid group. Conclusion SI can improve lipoprotein（a） and lipid peroxidation in ovariectomized rats. A continuous intervention with high-SI can reduce LDL- C to normal lever and retain integrity and ultrastructure of hepatocyte.

关 键 词：脂质过氧化 脂代谢 大豆异黄酮 电镜 去卵巢大鼠 

分 类 号：R285.5[医药卫生—中药学]