一种快速、经济、省时的施万细胞体外培养和纯化方法  

A FAST,ECONOMICAL AND SIMPLIFIED METHOD TO CULTURE AND PURIFY SCHWANN CELLS IN VITRO

作　　者：张雪宝[1] 曾园山[1] 陈穗君[1] 

机构地区：[1]中山大学中山医学院组织学胚胎学教研室神经科学研究室,广州510080

出　　处：《解剖学报》2007年第5期628-630,共3页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30270700);广东省社会发展攻关项目(2003C33808)

摘　　要：目的探讨一种快速、简单、经济的施万细胞(SCs)培养和纯化方法,为SCs体内移植研究提供稳定及可靠的细胞来源。方法选用SD乳鼠,取坐骨神经和臂丛神经,用单细胞双酶消化法和半植块单酶消化法培养SCs。结果用同样多的组织材料,半植块单酶消化法所获得的SCs比单细胞双酶消化法所获细胞至少多两倍,省时40min。免疫组织化学染色显示,获得的施万细胞96%以上呈S-100阳性。结论用半植块单酶消化法培养SCs,可以在短时间内获得足够数量和足够纯度的SCs。Objective To explore a fast, simplified and economical method for Schwann cells（SCs） cultured and purified in vitro. In this way, a stable and reliable cell sources can be provided in order to study SCs transplanted in vivo. Methods SCs cultures were prepared from the sciatic and brachial nerves of 3 to 5-day-old SD neonatal rats with double enzyme digestion method to acquire dissociated cells and one enzyme digestion method to acquire incomplete digested tissues. Results With the same number of neonatal rats, one enzyme digestion（hemi-explant） method acquired at least two times more SCs than double enzyme digestion（single cell） method did and saved at least 40 minutes. 96% of S-100 positive SCs cultured were shown by immunohistochemical staining. Conclusion When the one enzyme digestion method （hemi-explant） is used to culture SCs, sufficient SCs with qualified purity can be acquired in a short time.

关 键 词：施万细胞 细胞培养 单酶消化法 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]