黄檗(Phellodendron amuranse)叶片总RNA提取方法研究  

The method for RNA isolating from leaves of Phellodendron amuranse

作　　者：祖元刚[1] 王延兵[1] 王慧梅[1] 孙莲慧[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2007年第5期593-595,共3页Bulletin of Botanical Research

基　　金：国家重点基础研究发展规划项目(G19990160)

摘　　要：以黄檗(Phellodendron amuranseRupr.)叶片为材料,分别利用改进的盐酸胍法、Trizol法、CTAB法提取黄檗叶片总RNA,通过RNA产率、纯度、电泳图谱等分析,确立了1种从黄檗叶片中快速分离总RNA的方法。研究结果表明,改进盐酸胍法所提取的总RNA的A260/A280为1.928,28S和18S条带清晰谱图完整性好,而且具有产率高、时间短、成本低的特点,所提取的总RNA适用于mRNA分离、cDNA文库的建立、Northern杂交等分析。A method of rapid isolation of total RNA from leaves of Phellodendron amuranse Rupr. was developed. RNA was extracted by guanidine hydrochloride, Trizol regent, and CTAB, respectively with analysis the rate of yield, purity and electrophoretograms. The A260/A280 of RNA extracted by guanidine hydrochloride was 1. 928 with two clear bands of 28S and 18S and good integrity. The feather of RNA by this method was with less cost, fast, high purity and quantity, which can meet the demands of mRNA i- solation, cDNA library construction and Northern blotting.

关 键 词：黄檗 叶片 RNA 提取 

分 类 号：Q78[生物学—分子生物学]