南方红豆杉产紫杉醇内生真菌的分离鉴定  

Isolation and Identification of the Taxol-producing Endophytic Fungi from Taxus chinensis var.mairei

作　　者：郭晓静[1] 王俊鹏[2] 宋晓平[1] 周乐[3] 豆传娜[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨陵712100 [2]西北农林科技大学农学院,陕西杨陵712100 [3]西北农林科技大学理学院,陕西杨陵712100

出　　处：《西北植物学报》2007年第9期1874-1878,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30571402)

摘　　要：从南方红豆杉(Taxus chinensisvar.mairei)植株分离到内生真菌63株,其中24株经鉴定分属于19个种(属).真子囊菌纲的2个属2株,半知菌纲的14个属22株.真子囊菌纲的无毛毛壳属(Achaetomiumsp.)的1株为新发现种.半知菌纲的葡萄孢属(Botrytissp.)1株和无孢菌群(Mycelia sterlia)2株的培养液,经浓缩萃取,薄层层析检测,Rf值分别为0.404、0.404、0.401,与紫杉醇对照品Rf值一致,为南方红豆杉产紫杉醇内生真菌.Sixty-three strains of endophytes were isolated and obtained from the stems,leaves and roots of Taxus chinensis var. rnairei,of which 24 strains belonged to 19 genera（groups）according to their morphological characterization,respectively. There were 2 strains in genera of Ascomycotina and 22 strains in 14 genera of Deutermycotina. 1 strain in genera of Achaetornium sp. was a new species. 1 strain in genera of Deutermycotina and 2 strains in group of Mycelia sterlia were the taxol-producing endophytic fungi,which the Rf on thin layer chromatography （TLC） of the extracts from fermentation liquid were 0. 404,0. 404,0. 401,respectively,the value is close to that of sdanderd taxol.

关 键 词：南方红豆杉 紫杉醇 内生真菌 分离 鉴定 

分 类 号：Q946.8[生物学—植物学] Q93-331