CTC法结合体外受精比较不同方法对绵羊精子体外获能的影响  

Comparison of CTC Staining and in Vitro Fertilization among Different Ovine Sperm Capacitation Methods

作　　者：刘烈琴[1] 刘丑生[2] 郭勇[1] 倪和民[1] 张宏[1] 杨飞鸽[1] 刘云海[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206 [2]农业部全国畜牧兽医总站畜禽牧草种质资源保存利用中心,北京100094

出　　处：《中国畜牧兽医》2007年第8期31-34,共4页China Animal Husbandry & Veterinary Medicine

基　　金：北京市教育委员会科技发展计划重点项目(KZ200510020013);2006年度北京市教委"都市型现代农业关键技术的研究"项目部分资助

摘　　要：为比较不同获能方法对绵羊精子的获能效果,新鲜绵羊精液分别经肝素上游法(swim-up)、钙离子载体(IA,A23187)诱导法、Percoll离心法和Percoll离心后肝素孵育法进行精子获能的诱导后与体外成熟的绵羊卵母细胞进行体外受精,并利用金霉素荧光染色(chlortetracycline,CTC)法结合各自相应的体外受精结果对其获能效果进行检验。结果显示上游法处理后精子的获能比例和受精后的卵裂率均高于其它组,说明肝素上游法可以较好的诱导绵羊精子获能。In this experiment, the discrepancy was compared among the ＂Swim-up＂ , Induction by A23187, Percoll and Incubation after Percoll methods for ovine sperm capacitation in vitro with chlortetracycline fluorescence （CTC） staining and in vitro fertilization. The results showed that the rates of sperm capacitation and the cleavage treated by Swim-up method were higher than the others. It might be suggested that the Swim-up method is exactly suitable for the application of in vitro fertilization of sheep.

关 键 词：绵羊精子 获能 CTC 体外受精 

分 类 号：Q813[生物学—生物工程]