阿托伐他汀对醛固酮诱导心脏成纤维细胞内皮素A型受体mRNA表达的影响  

Effects of atorvastatin on the expression of endothelin subtype A receptor mRNA expression induced by aldosterone in cardiac fibroblasts

作　　者：武宇洲[1] 崔炜[1] 李淑琴[2] 张雷[3] 鲁静朝[1] 张冀东[1] 都军[1] 

机构地区：[1]河北医科大学第二医院河北省心脑血管病研究所心内科,石家庄050000 [2]河北医科大学病理生理教研室 [3]河北医科大学组织与胚胎学教研室

出　　处：《临床心血管病杂志》2007年第9期682-684,共3页Journal of Clinical Cardiology

摘　　要：目的:探讨阿托伐他汀(Ato)干预下,醛固酮(Ald)诱导新生大鼠心脏成纤维细胞内皮素A型受体(ET-AR)mRNA表达的变化及其机制。方法:采用胰酶消化法和差速贴壁分离法获取新生大鼠心脏成纤维细胞,应用RT-PCR测定不同条件下心脏成纤维细胞中的ET-AR mRNA的表达。结果:Ald(0.1μmol/L)可促进心脏成纤维细胞ET-AR mRNA的表达,提前给予Ato(1,10,100μmol/L)能剂量依赖性地抑制Ald的上述作用,同时Ato的这种抑制作用可被甲羟戊酸逆转。结论:Ato可抑制Ald诱导的心脏成纤维细胞ET-AR mRNA表达,发挥其抗心肌纤维化作用,其机制可能与甲羟戊酸代谢途径有关。Objective：To investigate the effect of atorvastatin on the expression of endothelin subtype A receptor （ET-AR） mRNA induced by aldosterone in cultured neonatal rat cardiac fibroblasts. Method：The cardiac fibroblasts of new born rat were cultured by trypsinogen and different speed adherence. The expression of ET-AR mRNA was detected by RT-PCR method. Result： ET-AR mRNA was up-regulated significantly in cardiac fibroblasts treated with aldosterone at 0. 1 μmol/L. Atorvastatin was down-regulated ET-AR mRNA expression with dose dependent whereas mevalonate reversed it. Conclusion： ET-AR mRNA expression in cardiac fibroblasts induced by aldosterone can be down-regulated by atovastatin, which is reversed by mevalonate.

关 键 词：醛固酮 阿托伐他汀 内皮素A型受体 心脏成纤维细胞 

分 类 号：R542.23[医药卫生—心血管疾病] R331.36[医药卫生—内科学]