基于叶绿体matK基因序列探讨10种石杉科石杉属植物的系统关系及分子鉴定  

Phylogeny relationship and molecular identification of ten Huperzia species(Huperziaceae) based on matK gene sequences

作　　者：姬生国[1] 潘胜利[1] 王峻[1] 霍克克[2] 

机构地区：[1]复旦大学药学院 [2]复旦大学生命科学学院,上海200433

出　　处：《中国中药杂志》2007年第19期1971-1975,共5页China Journal of Chinese Materia Medica

摘　　要：目的：利用叶绿体matK基因序列探讨10种石杉属植物的系统发育及分子鉴定。方法：分别从9种石杉属植物中提取总DNA，经扩增纯化后，用PCR产物直接测序。结果：对石杉属Huperzia Bernh．9个种的叶绿体matK基因进行了测序，序列长度为1589bp，并对10个种（亮叶石杉H.lucidula的matK基因序列从GenBank中下载）的marK序列进行比对，其中有35个系统发育信息位点及35个变异位点，以垂穗石松Lycopodiella cernua为外类群，利用MEGA 3．1软件计算种与种之间的遗传距离在1．59％～0．25％，并构建系统树，获得最简约树和邻接树。结论：小杉兰组和蛇足石杉组植物分别聚成一枝，构成姊妹群，其靴带支持率分别为98％和99％。长柄石杉与蛇足石杉分别聚在2个不同进化枝，二者间有19个碱基差异，遗传距离为1．21％。建议应将长柄石杉单独列为一个种。Objective： To study the phylogeny relationship and molecular identification of 10 species from Huperzia （Huperziaceae） based on marK gene sequences data. Method： Total DNA of nine species from Huperzia was extracted; marK gene sequence was amplified by PCR. PCR product was directly sequenced after purification. Result： The chloroplast marK gene nucleotide sequences from 9 species of Huperzia species were sequenced. The marK gene nucleotide sequences length was 1 589 bp. Analysis with Huperzia luciduia marK gene nucleotide sequences （download from C, enBank） and taking Lycopodiella cernua as outgroup, Maximum Parsimony, Neighbor-Joining analyses and genetic distances were conducted using MEGA 3.1 software. 35 variable sites and 35 parsimony informative sites have been found. Pairwise genetic distances among 10 species of Huperzia was 1.59%-0. 25%. Conclusion： The results were consistent with the taxonomy in morphological of Huperzia. But H. longipetiolata and H. serrata were resolved into in different clade. There are 19 different sites of marK gene sequences between H. longipetiolata and H. serrata, the genetic distances is 0. 121%. It is suggested H. longipetiolata should be as an independent species.

关 键 词：石杉属 marK基因 系统发育 分子鉴定 

分 类 号：S567.239[农业科学—中草药栽培]