超临界CO_2萃取鹿茸中的性激素研究  

Extraction of sex hormone from antler velvet with supercritical CO_2

作　　者：徐志红[1] 李淑芬[1] 王金宇[1] 周冉[1] 田松江[1] 

机构地区：[1]天津大学化工学院绿色合成与转化教育部重点实验室,天津300072

出　　处：《中国中药杂志》2007年第19期2000-2003,共4页China Journal of Chinese Materia Medica

摘　　要：目的:研究超临界CO2萃取技术提取鹿茸中性激素。方法:超临界CO2萃取鹿茸中的性激素并采用放射免疫法分析其含量,利用GC-MS,TLC和HPLC分析了萃取物中其他成分。结果:以85%乙醇为夹带剂,夹带剂∶原料=1(mL.g-1),萃取压力30 MPa,萃取温度65℃,总提取率为1.56%,雌二醇和孕酮的质量分数分别为3.07,776.18 ng.g-1。结论:采用超临界CO2萃取技术提取鹿茸中的性激素是可行的。Objective： To study the method of extraction of sex hormone from antler velvet with supercritical CO2. Method： Supercritical fluid extraction （SFE） was used to extract sex hormone from antler velvet and radioimmunoassay （RIA） was used to analyze the extracts. The chemical compositions in extracts were identified by GC-MS, TLC and HPLC, respectively. Result： The experimental results indicated that the extraction yield was 1.56% when 85% ethanol was used as co-solvent at temperature of 65 ℃ and extraction pressure of 30 MPa. Estradiol and progesterone in the extracts were 3.07, 776. 18 ng · g^-1 respectively. Conclusion： It is feasible to extract hormone from antler velvet with supercritical CO2.

关 键 词：超临界CO2 鹿茸 性激素 雌二醇 孕酮 

分 类 号：R284[医药卫生—中药学]