丹皮多糖-2b对大鼠糖尿病性白内障防治作用研究  

Protection of polysaccharides-2b from mudan cortex of Paeonia suffruticosa andr on diabetic cataract in rats

作　　者：赵根海[1] 沈业寿[1] 马金宝[1] 李峰[1] 史新强[1] 

机构地区：[1]安徽大学生化微生物研究所安徽省中药研究与开发重点实验室,安徽合肥230039

出　　处：《中国中药杂志》2007年第19期2036-2039,共4页China Journal of Chinese Materia Medica

基　　金：安徽省"十五"重大科技专项基金(01803011)

摘　　要：目的:观察丹皮多糖-2b对大鼠糖尿病性白内障的防治作用。方法:采用链尿佐菌素(STZ)联合完全弗氏佐剂(CFA)诱导大鼠糖尿病性白内障,裂隙灯观察各组晶体出现初次混浊的时间,比较晶体的混浊程度。比色法测定各组血清及晶体中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-pX)、过氧化氢酶(CAT)活力和丙二醛(MDA)含量以及晶体中Na+-K+-ATP酶活力、高分子质量蛋白和不溶性蛋白含量。结果:裂隙灯检查结果显示PSM2b有明显的延缓糖尿病性白内障的发生,减轻晶体混浊程度的作用。与模型组比,PSM2b各剂量组均能明显降低大鼠血清及晶体中升高的MDA水平,中、高剂量组可升高血清及晶体中SOD,GSH-pX,CAT水平,上调晶体Na+-K+-ATP酶活力,且剂量与效果呈正相关。PSM2b中、高剂量组对高分子质量蛋白及不溶性蛋白含量亦有较好调控作用。结论:PSM2b对大鼠糖尿病性白内障具有显著的防治作用。Objective： To observe the the protective effection of polysaccharides-2b of mudan cortex of Paeonia suffruticosa andr （PSM2b） on diabetic cataract. Method： The animal model of diabetic cataract in rats was induced by streptozotocin （STZ） and freund＇ s adjuvant complete （CFA）. The initial opacity occurrence time in lens was investigated with cranny lamp, and opacity degree of lens was compared too. The activities of glutathione peroxidase （ GSH-pX）, superoxide dismutase （ SOD）, catalase （ CAT）, the content of malonaldehyde （MDA） in serum and lens were detected. At the same time, the activities of Na^＋-K^＋ -ATPase, the content of macromolecular weight protein and infusibility protein in lens were detected too. Result： The results examinated by cranny lamp showed that PSM2b could significantly postpone the occurrence and alleviate opacity degree of lens. Compared with model group, every treatment group of PSM2b could lower the level of MDA, high and middle dose groups could increase the levels of SOD, GSH-pX, CAT in serum and lens in evidence, and enhance the activity of Na^＋ -K ^＋ -ATP ase. These indexes present favorable positive correlation between dose and effect. Conclusion： All these results demonstrated that PSM2b had apparently protective effection on diabetic cataract in rats.

关 键 词：丹皮 多糖 链尿佐菌素 完全弗氏佐剂 糖尿病性白内障 

分 类 号：R285.5[医药卫生—中药学]