鲜姜有效部位对单核细胞趋化蛋白和黏附分子表达的影响  

Influence of effective part of Zingiber officinal on expression of monocyte chemotactic protein-1 and adhesion molecules

作　　者：宋芸[1] 魏欣冰[1] 丁华[1] 程秀民[2] 

机构地区：[1]山东大学医学院药理学研究所,山东济南250012 [2]山东大学药学院,山东济南250012

出　　处：《中国中药杂志》2007年第19期2062-2065,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30271584);山东省自然科学基金项目(Y2003C13)

摘　　要：目的:研究鲜姜有效部位对离体培养的血管内皮细胞与单核细胞的黏附力及对单核细胞趋化蛋白和黏附分子表达的影响,探讨其保护血管内皮细胞的作用机制。方法:采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型,取健康大鼠每日灌服不同剂量的鲜姜有效部位(200,400,800 mg.kg-1)或阳性对照药洛伐他汀(40 mg.kg-1),取含药血清作为受试药物,用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力;逆转录聚合酶链反应(RT-PCR)测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平;酶联免疫吸附法(ELISA)测定MCP-1蛋白表达水平。结果:鲜姜有效部位能够显著降低内皮细胞与单核细胞的黏附力,并减低受损的血管内皮细胞表达ICAM-1,VCAM-1及MCP-1。结论:鲜姜有效部位可以降低由氧化损伤所引起的ECV-304细胞过度表达ICAM-1,VCAM-1及MCP-1,阻止单核细胞向内皮细胞黏附,从而保护血管内皮细胞免受损伤。Objevtive： To investigate the effect of effective parts of Zingiber officinal（EPZ） on the adhesion of ECV-304 cells with monocytes cultivated in vitro and on the expression of monocyte chemotactic protein-1 （ MCP-1 ） and adhesion molecules. Method： The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide （ H2O2 ）. Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 （ ICAM-1 ）, vascular cell adhesion molecule-1 （ VCAM-1 ） and MCP-1 mRNA expression in cells were detected by RT-PCR. MCP-1 protein expression were detected by ELISA. Result： EPZ could decrease the adhesion of monocytes with ECV-304 cells obviously. Meanwhile it could diminish the expression of ICAM-1, VCAM-1 and MCP-1 in injured ECV-304 cells. Conclusion： EPZ could inhibit H2 O2-induced ICAM-1, VCAM-1 and MCP-1 expression in ECV-304 and could inhibit the adherence of monocytes to endothelial cell, which may result in the protect effect in endothelial cells.

关 键 词：鲜姜有效部位 过氧化氢 细胞间黏附分子 血管细胞黏附分子 单核细胞趋化蛋白 

分 类 号：R285.5[医药卫生—中药学]