多房棘球绦虫重组BCG-EmⅡ/3疫苗促进小鼠脾细胞因子的分泌  

Cytokines secretion by splenocytes of mice was promoted by recombinant BCG-EmII/3 vaccine of Echinococcus multilocularis

作　　者：李文桂[1] 王鸿[1] 朱佑明[1] 

机构地区：[1]重庆医科大学第一附属医院传染病寄生虫病研究所,重庆400016

出　　处：《基础医学与临床》2007年第9期966-969,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30500423);教育部重点项目(205131);重庆市教委科研基金(KJ050313);重庆市科委科研基金(03-43-8);重庆市卫生局科研基金(03-2-099)

摘　　要：目的动态观察多房棘球绦虫重组BCG—EmⅡ／3疫苗免疫小鼠后脾细胞因子的变化。方法采用鼻腔内接种疫苗免疫BALB／C鼠，在免疫后0、2、4、6、8和10周各剖杀4只小鼠取脾，分离脾细胞，用EmAg或ConA刺激培养，收集脾细胞培养上清液，检测脾细胞培养上清液中的IL-2、IFN-γ、TNF-α和IL-4水平，同时设有PBS对照。结果疫苗接种组中IL-2、IFN-γ、TNF-α和IL-4分别在免疫后2～6、2～6、2—10和8～10周升高，分别在免疫后4、4、8和10周达最高水平。结论多房棘球绦虫重组BCG-EmⅡ／3疫苗在免疫早期（2～8周）即可诱导小鼠产生一个TH1型保护性免疫反应。Objective To dynamically observe changes of cytokines of splenocytes in mice by immunization with recombinant BCG-EmⅡ/3 vaccine of Echinococcus multilocularis （Em）. Methods BALB/C mice were intranasally vaccinated and then killed to get spleen on 0,2,4,6,8 and 10w of immunization respectively, splenocytes were isolated to culture with stimulation of EmAg or ConA, their supernatants were gathered to measure the level of IL-2,IFN-γ,TNF-α and IL-4 by ELISA Kits. Results In the groups of immunization, levels of IL-2, IFN-γ,TNF-α and IL-4 obviously increased on 2 -6,2 -6,2 - 10 and 8 - 10w respectively, reached the highest level on 4,4,8 and 10w respectively. Conclusion TH1 response was induced in mice by rBCG-EmⅡ3 vaccine by early immunization （2 - 8w）.

关 键 词：多房棘球绦虫 重组BCG—EmⅡ/3疫苗 细胞因子 

分 类 号：R392[医药卫生—免疫学]