兔动脉粥样硬化形成过程中血清NOS活性的变化  

Change of nitric oxide synthase activity in serum of atherosclerotic rabbit

作　　者：赵慧颖[1] 徐宝华[2] 马小欣[1] 

机构地区：[1]吉林大学第一临床医院心血管科,吉林长春130021 [2]大连市第三人民医院心内科,辽宁大连116033

出　　处：《基础医学与临床》2007年第9期1021-1024,共4页Basic and Clinical Medicine

摘　　要：目的研究兔动脉粥样硬化形成过程中血一氧化氮合酶(NOS)活性的变化及其作用。方法将30只长耳白兔随机分为3组:普通饮食组、高脂饮食组、球囊损伤+高脂饮食组,每组10只,3个月后以中国斑点蝰蛇毒和组胺触发使斑块破裂。生化监测血脂、血清NO、NOS及OH-水平,病理检查动脉硬化斑块。结果高脂饮食组、球囊损伤+高脂饮食组血脂水平明显增高;血清NO水平在药物触发及斑块破裂后明显减少;NOS活性及OH-水平在高脂喂养后明显升高,药物触发后明显减少。结论动脉粥样硬化早期NOS活性增强,内皮功能得到代偿;斑块处于不稳定状态时,NOS活性明显下降,内皮功能明显受损进入失代偿状态。Objective To find the changes and effects of nitric oxide synthase activity in atherosclerotic rabbit models. Methods Thirty rabbits were divided into 3 groups at random： control group, atherogenic diet group, ballooninjury ＋ atherogenic diet group. Three months later underwent pharmacological triggering with Chinese Russell＇ s viper venom and histamine to make the plaque rupture. Lipid concentrations and the levels of nitric oxide （NO） , NOS and hydroxy radical （OH） in the serum were obtained at different period, and pathologic changes were observed. Results In atherogenic diet group and balloon-injury ＋ atherogenic diet group, serum lipid concentrations markedly increased, but the levels of NO did not show any difference. The activity of NOS and OH levels were obviously increased after the atherogenic diet. But it reduced after pharmacological triggering and plaque rupture. Conclusion In early period of atherosclerosis, NOS activity up regulated and endothelium functions were compensated. But, When the atherosclerotic plaques were unstable, the activity of NOS descended obviously and endothelium function goes into incompensation.

关 键 词：一氧化氮合酶 动脉粥样硬化 斑块稳定性 

分 类 号：R543.5[医药卫生—心血管疾病]