模拟微重力对改良纤维蛋白胶软骨细胞复合物培养的影响  

The Effects of Simulated Microgravity on the Culture of Stabilized Fibrin-Chondrocyte Constructs

作　　者：吴华[1] 吴纪饶[2] 朱立新[3] 宇丽[4] 余磊[5] 

机构地区：[1]海南师范大学体育系,海口571158 [2]江西师范大学体育学院 [3]南方医科大学附属珠江医院骨科 [4]暨南大学医学院 [5]南方医科大学解剖教研室

出　　处：《中国运动医学杂志》2007年第5期547-551,共5页Chinese Journal of Sports Medicine

基　　金：广东省科技计划项目(编号:2003C34002)

摘　　要：目的:研究模拟微重力条件对体外构建工程化软骨的影响。方法:将兔软骨细胞种植到经抑肽酶和氨甲环酸改良的纤维蛋白胶支架材料上;设旋转培养组和静态对照组,体外培养3周,对复合物进行组织学观察和生化指标检测。结果:采用改良支架的复合物经体外培养3周,软骨基本保持了原有塑形;相对于静态培养组,旋转培养组DNA、GAG的合成量增加,并在第3周出现显著性差异。结论:经过改良的纤维蛋白胶支架能较长时间支持体外软骨组织的形成;旋转培养仪提供的模拟微重力环境可提高工程化软骨的质量,为实现关节软骨损伤的修复提供有效途径。Objective The purpose of the study was to explore the effects of simulated microgravity on the culture of stabilized fibrin-chondrocyte constructs. Methods The chondrocytes were seeded in the modified fibrin glue, containing appropriate concentration of antifibrinolysin （aprotinin and tranexamic acid）. The constructs were divided into two groups, rotating culture group （R） and static control group （S）. Two groups were cultured for three weeks. Group R was subjected to simulated microgravity （using rotating cell culture system, RCCS） and group S were cultured in 12--well plate. Gross observation, wet weight of both group samples were assessed histologically and immunohisto- logically. Results （1） Using modified scaffold, the original size and shape of the constructs were main- tained basically after three weeks of culture. （2） As compared with the static culture, the simulated microgravity produced more contents of DNA and GAG in group R, and the marked differences appeared at week 3. Conclusion （1） The modified fibrin gel scaffold had the potential to keep cartilaginous tissue formed in vitro for a long period of time. （2） The simulated microgravity by RCCS could improve the quality of tissue--engineered cartilage formed in vitro.

关 键 词：软骨组织工程 改良纤维蛋白胶 旋转培养仪 模拟微重力 

分 类 号：R318[医药卫生—生物医学工程]