虎纹捕鸟蛛毒素-I对全脑缺血再灌损伤大鼠海马组织中TNF凋亡通路的影响  

Effect of Huwentoxin-I on the TNF Apoptosis Pathway in Hippocampus of Rat with Global Cerebral Ischemia

作　　者：王一蓉[1] 毛海峰[2] 刘仁仪[3] 陈嘉勤[3] 

机构地区：[1]湖南体育职业学院 [2]江西宜春学院 [3]湖南师范大学体育学院

出　　处：《中国运动医学杂志》2007年第5期571-574,共4页Chinese Journal of Sports Medicine

基　　金：国家自然科学基金项目(编号:30671085);湖南省科技厅计划课题(编号:27040236)共同资助

摘　　要：目的:探讨注射虎纹捕鸟蛛毒素-I(HWTX-I)对全脑缺血模型大鼠神经保护作用的可能分子机制。方法:48只SD大鼠随机分为假手术组、非用药组和用药组,采用改良的Pulsinelli大鼠四血管阻断全脑缺血结合蛛网膜下腔置管模型,应用光镜、免疫组化方法观察各组大鼠海马CA1区锥体细胞Nissl染色形态学变化及海马组织中肿瘤坏死因子α(TNFα)、肿瘤坏死因子受体I(TNFRI)、TNF相关死亡结构域(TRADD)、Fas相关死亡结构域(FADD)、Caspase8等TNF凋亡通路相关因子蛋白表达水平的变化。结果:(1)用药组大鼠锥体神经元排列较整齐,略为疏散分布,而非用药组大鼠锥体神经元排列散乱,层次不完整,稀疏分布;(2)TNFα、TNFRI、TRADD、FADD、Caspase8阳性单位的表达值以非用药组最高,用药组次之,假手术组表达最低。结论:HWTX-I能明显减轻全脑缺血再灌损伤大鼠海马锥体细胞的损伤,并降低大鼠海马组织TNFα、TNFRI、TRADD、FADD、Caspase8蛋白水平表达,表明HWTX-I对全脑缺血再灌损伤大鼠海马神经细胞凋亡具有抑制作用,在一定程度上对全脑缺血再灌损伤有神经细胞保护作用。Objective To observe the effect of Huwentoxin-I （HWTX-I） on the protein expres- sions of related factors in TNF apoptosis pathway of hippocampus in rats with global cerebral ischemia,and investigate the possible neuroprotective mechanism of HWTX-I. Methods SD rats were randomly divided into sham-operation group, non-administered group and administered group. The model of improved Pulsinelli 4-vessel occluded global cerebral ischemic damage combined with the method of chronic intrathecal catheterization was adopted to observe the morphological change in py- ramidal neurons （Nissl staining） and detect the protein expressions of related factors of TNFα （tumor necrosis factor）, TNFRI （TNF receptor I）, TRADD （TNF receptor-associated death domain）, FADD （Fas-associated death domain）, and caspase 8 in TNF apoptosis pathway in the hippocampal CA1 region. Results （1） In＇ the administered group, pyramidal neurons array remained in order with less compact as compared with that in sham-operated group, however, those in non-administered group, pyramidal neurons failed to form clear layers, distributed sparsely in the whole CA1 region; （2） The statistics showed that the positive unit values of TNFa, TNFRI, TRADD, FADD and caspase 8 were the highest in the non-administered group, followed by the administered group and the sham-operated group. Conclusion HWTX-I has an inhibitory neuroprotective effects on the hippocampal neural apoptosis of rats with global cerebral ischemic reperfusion injury.

关 键 词：全脑缺血再灌 HWTX-I TNF凋亡通路 大鼠 

分 类 号：R96[医药卫生—药理学]