股部皮下埋藏睾丸对精子发生影响的实验研究  

An experimental study of the effect of burying testis in thigh pocket on spermatogenesis

作　　者：王达利[1] 王玉明[1] 魏在荣[1] 郑洪[2] 邓飞[2] 高振宇[3] 

机构地区：[1]遵义医学院附院整形外科,遵义563003 [2]遵义医学院附院病理科,遵义563003 [3]遵义医学院附院外科动物实验中心,遵义563003

出　　处：《中华整形外科杂志》2007年第5期419-421,共3页Chinese Journal of Plastic Surgery

基　　金：贵州省优秀科技教育人才专项基金项目（2005-65）

摘　　要：目的 探讨采用股部埋藏睾丸的方式修复阴囊皮肤缺损对睾丸精子发生的影响。方法 以育龄期贵州小型猪为实验动物，随机将雄性小型猪分为实验组和对照组。实验组动物手术切开阴囊制作股部埋藏睾丸动物模型，对照组手术处理同实验组但睾丸不移位。3个月后实验组、对照组随机各取2只行睾丸活检，观察睾丸的病理变化。然后将实验组、对照组6只分别与雌猪配对喂养3个月，观察有无生育，并在动物模型建立6个月后再次对所有实验组和对照组动物进行睾丸活检。结果 术后3个月和6个月实验组病理检查均发现睾丸曲细精管内各级生精细胞明显减少，未见成熟精子；而对照组睾丸曲细精管内各级生精细胞正常，可见成熟精子。术后3个月后，实验组动物与雌性动物配对喂养3个月无生育；而对照组生育正常。结论 猪股部埋藏睾丸的方法影响睾丸的精子发生，建议临床上最好不用此方法修复阴囊皮肤缺损，尤其是对希望保留生育能力的患者。Objective To explore the effect of burying testis in thigh pocket on spermatogenesis. Methods Guizhou miniature male pigs at child-bearing period were randomly divided to receive operation of scrotum incision and dissection with the testis burying in thigh pocket（experimental group） or without（ control group）. 3 months later, testis biopsy was performed on 2 pigs from each group for pathological examination . Then every male pig from both experimental （ n = 6） and control group （ n = 6） got a mating partner and lived together for 3 months. The fertility of the male pigs was observed. 6 months after operation, testis biopsy was performed again on all the animals from both the groups. Results Both at 3 months and 6 months after operation, the pathological examination showed the spermatogenic cells of all stage in contorted seminiferous tubules markedly decreased with no mature sperm in experimental group, while normal spermatogenic cells with mature sperm in control group. After the male pigs lived with mating parters for 3 months, no female pigs staying with the experimental group became pregnant, but the male pigs in control group had a normal fertility. Conclusions Burying testis in thigh pocket impedes spermatogenesis in the miniature male pig. So burying testis in thigh pocket is not recommended for patients with scrotum skin defect who wish to remain fertile.

关 键 词：阴囊 撕脱伤 精子发生 

分 类 号：R686[医药卫生—骨科学]