野牛草有性繁殖特性及其抗旱转录因子DREB的克隆  

Study on the Sexaul Reproductive Characteristic of Buchloe dactyloides and Cloning of DREB Transcription Factor Involved in Its Drought Resistance

作　　者：胡晓艳[1] 呼天明[1] 杨青川[1] 

机构地区：[1]西北农林科技大学动物科技学院草业科学系

出　　处：《草地学报》2007年第5期503-505,共3页Acta Agrestia Sinica

基　　金：科技部国际科技合作项目(2006DFA33630)

摘　　要：Buffalo grass (Buchloe dactyloides (Nutt.) Engelm.) is an extensive managing turf grass that has strong resistance against adverse environment. In the 20th century 40’s, this grass was introduced as the plant for water and soil conservation from US to Tianshui, Gansu Province and displayed good compatibilities. At present, the variety of buffalo grass is comparatively simplex in our country, the seed output is low and the major amount of seeds are dependent on the import from overseas, and the seed price is extremely expensive. The vegetative reproduction method is usually used to establish the lawn, but it requires a lot of time-consuming works and is not valid for the long-distance application. Therefore, it is a main research direction to breed new varieties with longer green time and higher seed output. In addition, fully use of the strong drought resistance characters of this plant resource and its molecular mechanism as well as the related gene separation and clone would enhance the the arid patience and compatibility of the plant for better perfomences on the desert prevention and control, ecological environmental protection, productivity, and ecological agriculture technologies in the loess plateau areas. The flower organ shape, blossom habit, acceptation of stigma, and the effects of different management regimes on the seed production of B. dactyloides were studied by this research. The result indicates that the flower organ is different greatly in the external shapes and blooming characteristics between male and female, but similar in florescence periods. The acceptation of female stigma is strongest in the latter 10 hours to blossom, and weaken gradually after 24 hours. In order to obtain the high yield and good quality of seed, the artificial wrap bag pollination should be applied in the beginning period of blooming of male plants and in the morning also. Fertilization and irrigation should be employed in the earlier earing period and the earing period of female plants, not suitabl during blooming pBuffalo grass （Buchloe dactyloides （Nutt.） Engelm. ） is an extensive managing turf grass that has strong resistance against adverse environment. In the 20th century 40＇s, this grass was introduced as the plant for water and soil conservation from US to Tianshui, Gansu Province and displayed good compatibilities. At present, the variety of buffalo grass is comparatively simplex in our country, the seed output is low and the major amount of seeds are dependent on the import from overseas, and the seed price is extremely expensive. The vegetative reproduction method is usually used to establish the lawn, but it requires a lot of time-consuming works and is not valid for the long-distance application. Therefore, it is a main research direction to breed new varieties with longer green time and higher seed output. In addition, fully use of the strong drought resistance characters of this plant resource and its molecular mechanism as well as the related gene separation and clone would enhance the the arid patience and compatibility of the plant for better perfomences on the desert prevention and control, ecological environmental protection, productivity, and ecological agriculture technologies in the loess plateau areas. The flower organ shape, blossom habit, acceptation of stigma, and the effects of different management regimes on the seed production of B. dactyloides were studied by this research. The result indicates that the flower organ is different greatly in the external shapes and blooming characteristics between male and female, but similar in florescence periods. The acceptation of female stigma is strongest in the latter 10 hours to blossom, and weaken gradually after 24 hours. In order to obtain the high yield and good quality of seed, the artificial wrap bag pollination should be applied in the beginning period of blooming of male plants and in the morning also. Fertilization and irrigation should be employed in the earlier earing period and the earing period of female plants, not suitabl durin

关 键 词：野牛草 有性繁殖 DREB 基因克隆 

分 类 号：S339[农业科学—作物遗传育种] Q785[农业科学—农艺学]