过氧化氢-鲁米诺流动注射化学发光法测定山奈酚  

Determination of Kaempferol by H_2O_2-Luminol Chemiluminescence Method

作　　者：李丽[1] 黄玉明[1] 

机构地区：[1]西南大学化学化工学院

出　　处：《西南大学学报（自然科学版）》2007年第9期68-71,共4页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市自然科学基金重点资助项目(2004BA7019)

摘　　要：在NaOH介质中，山奈酚对H2O2-luminol体系的化学发光有抑制作用，据此采用流动注射技术建立了测定山奈酚的化学发光新方法．在优化条件下，本法测定山奈酚的线性范围为2×10^-8～6×10^-6 g·mL^-1，检出限为1．8×10^-8 g·mL^-1，对1×10^-6 g·mL^-1山奈酚进行11次平行测定，其相对标准偏差为4．8％．研究了体系的化学发光光谱及紫外可见光谱特性，提出了发光反应可能的机理．将本法用于合成样品中山奈酚的测定，获得满意结果．A new flow injection chemiluminescence method based on the quench effect of kaempferol on the chemiluminescence reaction between luminol and H2O2 under alkaline conditions was developed for the determination of kaempferol. Under optimized conditions, the decrease of chemiluminescence intensity was linear with kaempferol conentrationn in the range of 2 × 10^-8 --6 × 10^-6 g/mL, the detection limit for kaempferol was 1.8 × 10^-8 g/mL and the relative standard deviation was 4. 8% （n=11） for 5 × l0^-8 g/mL kaempferol. Based on the studies on the spectra of CL and UV-vis absorption of the studied system, a possible CL mechanism has been proposed. The method was applied to the determination of kaempferol in synthetic samples with satisfactory results.

关 键 词：化学发光 山奈酚 过氧化氢 鲁米诺 

分 类 号：O657.39[理学—分析化学]