培养基质对丛枝菌根(AM)真菌生长发育的影响  

Effects of culture substrates on development of arbuscular mycorrhizal fungi

作　　者：陈宁[1] 王幼珊 蒋家珍[3] 杨延杰[1] 林多[1] 仇宏伟[1] 张美庆[2] 

机构地区：[1]青岛农业大学园艺学院,青岛266109 [2]北京市农林科学院植物营养与资源研究所,北京100089 [3]中国农业大学理学院,北京10094

出　　处：《农业工程学报》2007年第9期205-207,共3页Transactions of the Chinese Society of Agricultural Engineering

基　　金：国家自然科学基金资助项目(30070028);农业部引进项目(201058);莱阳农学院博士基金(630306)

摘　　要：温室条件下,以盆栽培养方法研究不同培养基质对丛枝菌根(Arbuscular Mycorrhizae,AM)真菌Glomus mosseae生长发育的影响。结果表明:不同理化性质的基质对菌根共生体生长发育的影响不同,综合考虑菌根长度、根外菌丝量及孢子数3项指标,以沙土混合物(体积比3∶1)对G.mosseae菌剂的生长发育最为有利。宿主植物菌根长度及根中的可溶性糖浓度与根外孢子数有正相关关系,而宿主植物中磷浓度与菌根真菌的生长发育也有类似的关系。说明培养基质的养分状况、水分状况、通气状况等诸多因素都会影响菌根共生体的建立和发展。宿主植物的菌根长度、根中可溶性糖浓度以及宿主植物磷浓度对菌根真菌的生长发育有显著影响。因此,工厂化AM菌剂生产中,应以沙土混合物(体积比3∶1)为生产G.mosseae菌剂的培养基质。Four different culture substrates（mixing sand, fine sand, zeolite, mixture of sand and soil） on the growth of arbuscular mycorrhizal fungus Glomus mosseae were studied. The results showed that different substrates with different physical and chemical properties influence the development of Glomus mosseae. On the basis of the length of colonized roots, length of external hyphae and spore numbers, the mixture of sand and soil（v/v 3 ： 1） achieved the greatest mycorrhizal development among the four culture sub- strates tested in association with G. mosseae. The length of colonized roots and the concentration of soluble sugar in roots were signifi- cantly and positively correlated with spore numbers in substrate. The concentration of P in host plant had the same relationship with the spore numbers. The results suggest that many factors of culture substrate such as nutrient, humidity, air capacity of soils and so on influence the establishment and development of host plant-fungi combinations. The length of colonized roots, the concentration of soluble sugar in roots and the concentration of P in host plant have great influence on the mycorrhizal development.

关 键 词：培养基质 丛枝菌根(AM)真菌 侵染率 菌丝量 孢子数 

分 类 号：Q93-335[生物学—微生物学]