间日疟原虫裂殖子表面蛋白3α基因分型及序列分析  

Genotyping and Sequence Analyzing of Merozoite Surface Protein-3α

作　　者：张山鹰[1] 肖方震[1] 许龙善[1] 黄江宏[1] 

机构地区：[1]福建省疾病预防控制中心,福建医科大学教学基地,福州350004

出　　处：《海峡预防医学杂志》2007年第4期7-9,共3页Strait Journal of Preventive Medicine

基　　金：福建省自然科学基金计划项目(项目编号:Z0516066)

摘　　要：[目的]分析间日疟原虫裂殖子表面蛋白3α(PvMSP-3α)基因多态性。[方法]套式PCR扩增PvMSP-3α基因,测序,进行序列分析。[结果]22个PvMSP-3α基因型中Ⅰ型的比例最大为81.8%(18/22),Ⅲ型分别为18.2%(4/22),未发现Ⅱ型以及混合条带。序列分析表明PvMSP-3α存在大量的核苷酸缺失或插入。[结论]PvMSP-3α存在广泛多态性。[Objective] To analyze the polymorphism of Plasrnodiurn vivax merozoite surface protein-3α （PvMSP-3α ）. [-Methods] Nested PCR was used to amplify PvMSP-3α , the PCR product of PvMSP-3α was purified and sequenced directly. The sequence was analyzed by bio-software. [Results] In 22 field isolates, the percentage of Genotype Ⅰ and Ⅲ of PvMSP-3a were 81.8% （18/22）and 18. 2 % （4/22）respectively. Genotype Ⅱ and multiple infection were not found. Sequence analysis showed that the sequence of PvMSP-3α existed a large number of amino acid deletions and substitutions. [Conclusion] PvMSP-3α are highly diverse.

关 键 词：间日疟原虫 裂殖子表面蛋白3α 基因分型 疟疾 

分 类 号：R382.3[医药卫生—医学寄生虫学]