熟地黄药材质量标准的研究  

Study on quality standard of Radix Rehmanniae Praeparata

作　　者：刘明[1] 李更生[1,2] 王慧森[1] 

机构地区：[1]河南省中医药研究院中药研究所 [2]北京中医药大学中药学院,北京100102

出　　处：《药物分析杂志》2007年第9期1311-1313,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家中医药局青年基金项目(95Y3411/95C018);河南省自然科学基金项目(0511043800)

摘　　要：目的:建立熟地黄药材的质量标准。方法:采用 TLC 法对熟地黄药材进行定性鉴别;用高效液相色谱法测定地黄苷 A和地黄苷 D 的含量。薄层色谱使用硅胶 G 薄层板,以三氯甲烷-甲醇-水(12:8:1)为展开剂,以5%香草醛浓硫酸溶液为显色剂;液相色谱采用 Inertsil NH_2色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-水(75:25),流速1 mL·min^(-1),检测波长203 nm,柱温40℃。结果:TLC 色谱系统中,地黄苷 A 和地黄苷 D 分离效果良好,可以用于熟地黄药材的鉴别;HPLC 中地黄苷 A 和地黄苷 D 进样浓度分别在0.064～0.320 mg·mL^(-1)和0.044～0.220 mg·mL^(-1)范围内线性关系良好,r 值分别为0.9999和0.9995,平均加样回收率(n=6)分别为97.3%和98.0%。结论:所建立的方法可准确地进行定性、定量分析,可用于熟地黄药材的质量控制。Objective：To establish the quality standard of Radix Rehmanniae Praeparata. Methods：The TLC and HPLC methods were used for qualitative identification and quantitative determination of rehmannioside A and D. TLC condition：Developer of selection was chloroform- methanol- water（ 12： 8：1 ）,5% vanillin sulfuric acid solution as coloration using silica gel G; HPLC ： The separation was performed on a Inertsil NH2 column （250 mm × 4. 6 mm, 5 μm） ,using the mobile phase of acetonitrile -water（75：25 ）with a flow rate at 1.0 mL · min^-1 ,the detection wavelength was set at 203 nm and column temperature was 40 ℃. Results：The identified characteristics of rehmannioside A and D were distinct and Radix Rehmanniae Praeparata could be identified by TLC;In the system of HPLC ,the calibration curves of rehmannioside A and D had a good linear relation respectively in the ranges of 0. 064 - 0. 320 nag · mL^-1 （r =0. 9999） and 0. 044 - 0. 220 mg · mL^-1 （ r = 0. 9995 ）. The average recoveries （ n = 6 ） were 97. 3% and 98. 0% respectively. Conclusion：The methods can be used for the quality control of Radix Rehmanniae Praeparata.

关 键 词：熟地黄 地黄苷A 地黄苷D 薄层色谱 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]