兰索拉唑脂质体包封率的测定  

Determination of entrapment efficiency of lansoprazole liposomes

作　　者：周臻[1] 邓英杰[1] 杨静文[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《药物分析杂志》2007年第9期1459-1461,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立兰索拉唑脂质体包封率的测定方法。方法:利用透析法分离脂质体和游离药物,采用紫外分光光度法测定兰索拉唑脂质体的包封率,在284 nm 处测定兰索拉唑脂质体中的含药量。结果:透析平衡时间为10 h,兰索拉唑浓度在2.1～10.5μg·mL^(-1)范围内线性关系良好(r=0.9998);该方法的平均游离药物回收率(98.5%～99.9%)符合要求;高、中、低3种浓度的日内和日间精密度试验的 RSD 均小于1%;透析24 h 内无渗漏,重现性好。结论:本法便捷、准确,适用于脂溶性药物兰索拉唑脂质体包封率的测定。Objective：To establish a method for determination of the entrapment efficiency of lansoprazole liposomes. Methods：The entrapment efficiency of lansoprazole liposomes was determined by dialysis method with UV for analyzing the amount of lansoprazole. Results ：The equilibrium time was 10 h. The calibration curve was linear（ r =0. 9998） with in the range of 2. 1 -10. 5 μg · mL^-1 for lansoprazole. The recoveries of the method were 98.5% -99.9%, and the RSDs of the intra -day and inter -day were less than 1%. The leaking percentage in 24 h was neglectable in the process of the dialysis. Conclusion： Dialysis method is convenient and accurate. It is suitable for determining the entrapment efficiency of liposomes of hydrophobic drugs such as lansoprazole.

关 键 词：兰索拉唑 脂质体 透析法 包封率 

分 类 号：R917[医药卫生—药物分析学]