草原兔尾鼠和昆明白小鼠卵透明带3 DNA疫苗免疫不育效果的研究  

THE IMMUNOCONTRACEPTIVE EFFECTS OF LAGURUS LAGURUS AND MUS MUSCULUS ZONA PELLUCIDA 3 DNA VACCINE

作　　者：张钰[1] 李轶杰[1] 刘菲[1] 陈蓉[2] 郭继华[3] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,乌鲁木齐830046 [2]新疆医科大学基础医学院,乌鲁木齐830054 [3]新疆空军总医院,乌鲁木齐830000

出　　处：《分子细胞生物学报》2007年第5期301-308,共8页Journal of Molecular Cell Biology

基　　金：国家自然科学基金(No.30360062);新疆教育厅创新群体资助项目(No:XJEDU2004G02)。

摘　　要：为探讨不同种类小鼠卵透明带3(zp_3)的免疫不育效果,并筛选高效且具有物种特异性的DNA抗生育疫苗,本研究选择草原兔尾鼠卵透明带3(Lzp_3)和昆明白小鼠卵透明带3(Mzp_3)构建不同的DNA抗生育疫苗表达载体,pcDNA3-mzp_3(pcD-M)、pcDNA3-lzp_3(pcD-L)、pcDNA3-aat- comp-mzp_3(pcD-ACM)和pcDNA3-aat-comp-lzp_3(pcD-ACL)分别免疫NIH小白鼠。研究中用水动力转染技术取代传统的Hela细胞真核转染检测小鼠体内真核表达情况,结果表明四种质粒均能在小鼠肝脏中进行表达:ELISA图示表明重组质粒均能使小鼠激发较高水平的特异性抗体;抗生育结果表明四种DNA疫苗均具有免疫不育的效果(P<0.05),其中pcDNA3-aat-comp-mzp_3(pcD-ACM)的免疫效果最好(P<0.01);卵巢病理切片H.E染色结果显示pcD-M和pcD-L组卵巢结构与对照小鼠区别不大,而pcD-ACL和pcD-ACM组卵巢结构发生病理性变化。结果初步说明草原兔尾鼠和昆明白小鼠卵透明带3对NIH小白鼠均有抗生育效果,但不具有物种特异性。To investigate the immunocontraception effects of different kinds of mice and seek the relatively effective species-specific DNA vaccines, we constructed four corresponding immunocontraceptive recombinant plasmids： pcDNA3-mzp3 （pcD-M）, pcDNA3-1zp3 （pcD-L）, pcDNA3-aat-compmzp3 （pcD-ACM） and pcDNA3-aat-comp-lzp3 （pcD-ACL） using zona pellucida 3 gene of Lagurus lagurus （Lzp3） and Mus musculus （Mzp3） respectively to immunize NIH mice. With the introduction of hydrnamic transfection instead of traditional Hela cell transfection to study the genes expression in vivo, the results indicated that all four recombinants could be expressed in livers of mice; Histogram pattern of ELISA showed that all of the recombinants in mice could elicit high quantity and lasting specific anti-ZP3 antibody; Antifertility experiment showed that Mzp3 and Lzp3 groups both enhanced sterile effects （P〈O.05）, especially the group of pcD-ACM had a significant difference compared with control group （P〈0.01）. Histology of ovarian sections demonstrated that pcD-M and pcD-L groups had no disruption of follicular development while pcD-ACL and pcD-ACM did the opposite. The present studies proved that L. lagurus zona pellucida 3 gene （Lzp3） and M. musculus zona pellucida 3 gene （Mzp3） had immunocontraception effects and primarily presumed that they didn＇t possess species specificity.

关 键 词：草原兔尾鼠 昆明白小鼠 卵透明带3 免疫不育 物种特异性 

分 类 号：Q789[生物学—分子生物学]