甘丙肽对PC12细胞和B104细胞增殖的不同影响  

DIFFERENT EFFECT OF GALANIN ON THE PROLIFERATION OF PC12 AND B104 CELLS

作　　者：程爽[1] 袁崇刚[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《分子细胞生物学报》2007年第5期315-322,共8页Journal of Molecular Cell Biology

摘　　要：本实验运用PC12细胞和B104细胞对甘丙肽(GAL)在神经增殖上的作用进行了研究。运用RT-PCR方法检测GAL及其受体在PC12细胞和B104细胞中的表达:运用MTT法检测GAL及其受体激动剂、拮抗剂对两种细胞增殖的影响。结果显示:PC12细胞表达所有三种GAL受体(GalRs),而不表达GAL;B104细胞表达GAL及两种受体GalR2和GalR3,而不表达GalR1;GAL及其受体激动剂GAL1-11和GAL2-11能够明显地抑制PC12细胞增殖.却会明显促进B104细胞的增殖。这些效应皆可被非特异性GAL受体拮抗剂M35所阻断。结果说明,GAL可以通过其受体影响细胞的增殖,并且不同受体可能介导不同的作用。The effect of galanin （GAL） on neural proliferation was studied in this article using PC12 and B104 ceils. RT-PCR was used to determine the expression of galanin and its receptors in both ceils. MTT analysis method was employed to detect the effects of galanin and the agonist, antagonist of galanin receptors on the proliferation of both ceils. Results showed that PC12 ceils expressed mRNAs for all the three galanin receptors （GalRs） but not for galanin, while B104 cells expressed galanin, GalR2, GalR3 except GalR1. In addition, galanin and two receptor agonists, GAL1-11 and GAL2-11, could inhibit the proliferation of PC12 ceils but activated the proliferation of B104 ceils significantly. Moreover, the influences could be blocked by M35, a nonspecific antagonist of galanin receptors. It suggested that GAL can affect cell proliferation via GalRs, but the different galanin receptors might mediate different cell functions.

关 键 词：甘丙肽 甘丙肽受体 细胞增殖 PC12 B104 

分 类 号：Q42[生物学—神经生物学]