实验性高血压大鼠左心室肥大心肌细胞中黏着斑激酶的表达  

Expression of focal adhesion kinase in cardiac myocytes of hypertrophic ventricle

作　　者：李展宇[1] 易先平[1] 钟玲[1] Fa-qian Li 周文英[1] 曹婉维[1] 郑燕璇[1] 王晓鸿[1] 王亚南[1] 

机构地区：[1]中山大学附属第五医院病理科, 珠海519000 [2]Cardiovascular Research Institute,University of South Dakota,Sioux Fails,SD,U S A

出　　处：《中华病理学杂志》2007年第10期677-680,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金(30470696)

摘　　要：目的研究黏着斑激酶(FAK)在高血压心室肥大发生发展机制中的作用。方法以特发性高血压心力衰竭(SHHF)大鼠为研究对象,通过免疫荧光标记、共聚焦显微镜及 Western blot等方法,检测不同月龄 SHHF 大鼠左心室心肌细胞中 FAK 的表达及其分布。结果实验组 SHHF 大鼠与对照组 WKY 大鼠比较,FAK 的表达在2月龄时无明显变化(50.5±6.9比49.8±5.0,n=6,P>0.05),而6、12、18月龄的 SHHF 大鼠心肌细胞中,FAK 表达明显增加(130.6±3.0比47.3±1.3,144.7±5.4比46.4±3.1,141.4±9.8比48.5±2.2,各组 n=6,P<0.05),并且聚集在心肌细胞两端的闰盘和细胞核等部位。结论在心肌肥大的发展过程中,FAK 表达增加并有闰盘及核内的聚积,提示 FAK 与心肌肥大关系密切,可能参与了心肌肥大细胞信号转导的调控。Objectives To investigate the role of focal adhesion kinase （FAK） in the pathogenesis of cardiac hypertrophy induced by hypertension. Methods Using immunofluorescent labeling, confocal microscopy and Western blotting, the expression and subcellular localization of FAK in the cardiac myocytes of left ventricle were determined in 2, 6, 12, and 18 month-old rats with spontaneously hypertensive heart failure （SHHF） along with age-matched control Wistar-Kyoto （WKY） rats. Results There was no significant difference of FAK expression between 2 month-old SHHF and WKY rats（50. 5±6.9 vs. 49. 8 ± 5. 0,n =6,P 〉0. 05）. In contrast with the control groups, the expression of FAK significantly increased in 6, 12 and 18 month-old SHHF rats （ 130. 6 ± 3.0 vs. 47.3 ± 1.3,144. 7 ± 5.4 vs. 46.4 ± 3. 1, 141.4 ± 9. 8 vs. 48.5 ± 2.2, each groups n = 6 ,P 〈 0. 05 ） with FAK protein primarily cumulated in the intercalated disks and nuclei. Conclusions FAK may play a role in the cell signaling transduction leading to cardiac hypertrophy, presumably through regulations of hypertrophic gene transcription and RNA processing.

关 键 词：黏着斑激酶1 高血压 心肌疾病 衔接蛋白质类 信号转导 

分 类 号：R544.1[医药卫生—心血管疾病] R541.3[医药卫生—内科学]