卷柏属药用植物的HPLC指纹特征研究  

Study on HPLC fingerprint characteristics of Selaginella plants

作　　者：范晓磊 万定荣[2] 叶丛进[2] 陈科力[2] 

机构地区：[1]武汉市药品检验所,湖北武汉430012 [2]湖北中医学院

出　　处：《中国中药杂志》2007年第20期2102-2106,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30470193)

摘　　要：目的:建立卷柏属10种药用植物的HPLC指纹特征分析方法,对各个种的区别及这些种的相似性从所含化学成分的角度进行综合评价,为这10种药用植物的鉴定、品质分析及亲缘关系评价提供依据。方法:采用HPLC获得的色谱数据转化为HPLC指纹谱,据此对各样品进行鉴定与分析。结果:卷柏属10种药用植物均显示了它们明显的共性和各自的指纹特征;部分种在不同采收期、不同生境及不同产地的样品在指纹谱上存在一定差异。结论:该属10种药用植物具有2个重要的共有峰,5个峰的指纹数据可以作为主要的指纹特征数据,这些数据对于判断各种间亲缘关系的疏密,评价药材品质具有重要的参考价值。Objective：To establish a method to analyze HPLC fingerprint characteristics of 10 plants from Selaginella.Method： HPLC was applied for establishment of fingerprints,which were used to evaluate and distinguish the different species of Selaginella.Result： The different species from Selaginella showed different HPLC fingerprint characteristic.The samples of the same species but collected in different period,different environment or different locations showed certain differece in fingerprints.Conclusion： 2 important mutual fingerprint peaks were found in the 10 plants of Selaginella species and 5 peaks can be used as ＂main fingerprint peaks＂.The dates of these peaks can used for assessment of phylogenetic relation among species and evaluation of quality.

关 键 词：卷柏属 HPLC 指纹特征 

分 类 号：S567.239[农业科学—中草药栽培]