补肾益气和血方对胚泡着床障碍小鼠子宫内膜P21表达的影响  

Effect of Bushen Yiqi Hexue recipe on endometrial P21 expression of embryo implantation dysfunctional mice

作　　者：黄冬梅[1] 黄光英[1] 徐丽君[1] 张明敏[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,湖北武汉430030

出　　处：《中国中药杂志》2007年第20期2152-2155,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30171193)

摘　　要：目的:观察补肾益气和血方对胚泡着床障碍小鼠着床部位子宫内膜中周期素依赖的蛋白激酶抑制物P21和增殖细胞核抗原(proliferation cell nuclear antigen,PCNA)表达的影响。方法:采用免疫印迹法观察胚泡植入[即妊娠第6天(pregnancy day 6,Pd6),Pd7和Pd8]时P21在整个子宫中的表达,采用免疫组化法进一步定位观察着床部位子宫内膜中PCNA和P21的表达。结果:与对照组相比,模型组子宫内膜PCNA和P21的出现和消失在时间和空间上均明显滞后;补肾益气和血方能促进PCNA和P21及时有序地表达或消失,从而改善胚泡着床障碍小鼠子宫内膜的蜕膜反应。结论:补肾益气和血方可通过促进子宫内膜的蜕膜反应来改善胚泡着床,其作用机制与其促进子宫内膜PCNA和P21时空有序地表达和消失密切相关。Objective：To observe the effect of Bushen Yiqi Hexue recipe on endometrial P21 and PCNA expression of embryo implantation dysfunctional mice.Method： The expression of P21 protein was analyzed by means of immunoblot and immunohistochemical method.The expression of PCNA protein was analyzed by means of immunohistochemical method.Result： The expression and disappearance of P21 and PCNA protein in endometrium of embryo implantation dysfunctional mice lagged behind in a temporal and cell specific manner.Bushenyiqihexue recipe could improve the expression of P21 and PCNA protein in a temporal and cell specific manner,and then improve the decidual reaction of endometrium of embryo implantation dysfunctional mice.Conclusion： Improving the expression of P21 and PCNA protein in endometrium and eventually regulating endometrium decidual reaction is one of the mechanisms of improved embryo implantaion by Bushen Yiqi Hexue recipe.

关 键 词：补肾益气和血方 胚泡着床障碍 蜕膜反应 PCNA P21 

分 类 号：R285.5[医药卫生—中药学]