茅莓总皂苷对局灶性脑缺血再灌注大鼠脑水肿及血-脑脊液屏障变化的影响  

Effect of total saponins of Rubus parviflolius(TSRP) on change of hydrated amount and blood-brain barrier in rats during focal cerebral ischemic/reperfusion

作　　者：王继生[1] 邱宗荫[1] 李惠芝[1] 夏永鹏[1] 周成林[1] 

机构地区：[1]重庆医科大学药学院,重庆400016

出　　处：《中国中药杂志》2007年第20期2166-2169,共4页China Journal of Chinese Materia Medica

基　　金：重庆市重大科技攻关项目[渝科发计字(2004)27号]

摘　　要：目的:研究茅莓总皂苷(TSRP)对局灶性脑缺血再灌注大鼠脑水肿及血脑屏障通透性的影响。方法:采用线栓法制备大鼠局灶性脑缺血再灌注模型,在大鼠脑缺血2 h再灌注6,24,48,72 h分别测定脑含水量和伊文思蓝含量的变化及茅莓总皂苷各剂量组的影响。结果:与模型组比较茅莓总皂苷各剂量组均能不同程度降低脑含水量和伊文思蓝含量。结论:茅莓总皂苷各剂量组显著降低缺血2h再灌注6,24,48,72 h脑水肿和其对血脑屏障通透性,这可能是其减轻I/R时脑水肿的机制之一。Objective：To explore the effects of total saponins of Rubus parviflolius（TSRP） on brain edema and blood brain barrier in rats.Method： The model of local cerebral ischemia was established in rats by reversible inserting a nylon thread into the anterior cerebral artery through the internal carotid artery brain hydrated amount and content change of Evan＇s blue（EB）in cortex subjected to 2h middle cererbral artery occlusion（MACO） followed by 6 h,24 h,48 h,72 h reperfusion and effect of TSRP.penetrability of blood brain-barrier（BBB） the index includes brain hydrated amount and penetrability of blood brain-barrier BBB.Result： Compared with I/R group.Both brain hydrated amount and the EB content decreased significantly in TSRP groups on the 6 h,24 h,48 h,72 h of reperfusion after 2 hour of cerebral ischemia induced by MACO mode1.Conclusion： TSRP could decrease brain hydrated amount and markedly lower permeability of blood-brain barrier subjected to 2 h MACO followed by 24 h reperfusion,and this may be a mechanism of TSRP alleviating brain edema during I/R.

关 键 词：茅莓总皂苷 脑缺血/再灌注 脑水肿 血脑屏障 

分 类 号：R285.5[医药卫生—中药学]