正交试验优选猪苓多糖长循环脂质体制备工艺的研究  

Optimizing preparation technique of Polyporus umbellatus polysaccharides long circulating liposomes by orthogonal test

作　　者：汪继红[1] 杨瑞[1] 范巧娜[1] 王凯平[1] 胡明慧[1] 

机构地区：[1]华中科技大学同济医学院药学院,湖北武汉430030

出　　处：《中草药》2007年第10期1484-1487,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的研究猪苓多糖长循环脂质体的处方和制备工艺。方法采用逆相蒸发法制备脂质体。以紫外分光光度-Sephadex法测定脂质体中猪苓多糖的包封率和载药量,采用单因素和正交试验法优化猪苓多糖长循环脂质体的处方和制备工艺。结果优化后的脂质体平均包封率为98.24%,平均载药量为27.20%。结论脂质体制备工艺合理,紫外分光光度-Sephadex法测定猪苓多糖长循环脂质体包封率准确性高,重现性好,简便易行。Objective To study the processing techniques for prescription and preparation of Polyporus umbellatus polysaccharides long circulating liposomes （PUPS LCLs）. Methods The liposomes were prepared by the reverse evaporating method. The main influencing factors were identified by single factor analysis, then the techniques of the prescription and preparation of liposomes were optimized. The entrapment efficiency and the carrying amount were determined by UV-Sephadex method. Results After optimized, the average entrapment efficiency of liposomes was 98.24% and the average carrying amount was 27.20%. Conclusion Preparation method of PUPS LCLs is available. UV-Sephadex method is simple and suitable for quality control of PUPS LCLs with desirable reproducibility and the results are reliable.

关 键 词：猪苓多糖 长循环脂质体 逆相蒸发法 正交试验法 制备 

分 类 号：R283.1[医药卫生—中药学] R286.02[医药卫生—中医学]