人参皂苷肠道代谢物诱导人肝癌BEL-7402细胞凋亡  

Apoptosis induced by a novel intestinal metabolite of ginseng saponin in human hepatocellular carcinoma BEL-7402 cells

作　　者：明艳林[1] 郑志忠[2] 陈良华[2] 童庆宣[2] 

机构地区：[1]厦门大学肿瘤细胞与分子生物学教育部重点实验室,福建厦门361005 [2]厦门华侨亚热带植物引种园天然产物研发中心,福建厦门361002

出　　处：《中草药》2007年第10期1511-1514,共4页Chinese Traditional and Herbal Drugs

基　　金：厦门市科技创新基金重点项目(3502Z20062008)

摘　　要：目的探讨一种新的人参稀有皂苷20-O-(β-D-吡喃葡萄糖)-20(S)-原人参二醇皂苷(IH-901)对人肝癌BEL-7402细胞的增殖抑制效果和诱导凋亡作用。方法以0、6.25、12.5、25、50、75、100μmol/L的IH-901作用于体外培养的BEL-7402细胞,通过MTT法检测IH-901对细胞生长的抑制作用,相差显微镜观察细胞形态变化,AO/EB的荧光核染色观察凋亡细胞形态,流式细胞术检测细胞周期和细胞凋亡。结果MTT实验显示IH-901抑制BEL-7402细胞的生长,呈时间及浓度依赖的方式;显微镜下观察到典型的凋亡形态变化和生化特征:细胞固缩,核染色质凝聚,出现凋亡小体;AO/EB的荧光核染色后,观察到典型的凋亡细胞;流式细胞仪可以检测到凋亡峰,且细胞被阻滞在G0/G1期。结论IH-901能通过诱导细胞凋亡的方式有效地抑制人肝癌BEL-7402细胞的生长,稀有人参皂苷IH-901可能成为一种潜在的抗肝癌药物。Objective To investigate the effects of 20-O-（β-D-glucopyranosyl）-20（S）-protopanaxadiol （IH-901） on the human hepatocellular carcinma BEL-7402 cells in terms of inhibition of proliferation and induction of apoptosis. Methods BEL-7402 Cells in cultural medium in vitro were given 0, 6.25, 12.5, 25, 50, 75, and 100 μmol/L IH-901. The inhibitory rate of cells was measured by MTT assay, morphology of cell apoptosis was observed by AO/EB fluorescent staining, cell apoptotic rate and cell phase were detected by flow Cytometry （FCM）. Results The results indicated that IH-901 could inhibit the proliferation of BEL-7402 cells significantly. The suppression was both in a time- and dose-dependent manner. Morphological examination of IH-901-treated samples showed cells with chromatin condensation, cell shrinkage, and all typical characteristics of apoptotic cells. Marked morphological changes of cell apoptosis was observed very clearly by AO/EB fluorescent staining. The increase in the apoptotic sub-G1 fraction in a concentration-dependent manner and cell cycle arrest at the G0/G1 phase were detected by FCM. Conclusion The results demonstrate that IH-901 dramatically suppresses BEL-7402 cell growth by inducing programed cell death. These results may provide a pivotal mechanism for the use of IH-901 in the prevention and treatment of human hepatocellular carcinoma BEL-7402 cells, the antiproliferation, and the apoptosis .

关 键 词：20-O-(β-D-吡喃葡萄)-20(S)-原人参二醇皂苷(IH-901) 人肝癌BEL-7402细胞 增殖 细胞凋亡 

分 类 号：R286.91[医药卫生—中药学]