胚胎环境下动脉导管、肺小动脉和主动脉钙离子浓度变化的体外实验研究  

Changes of intracellular calcium concentration in fetal lamb ductus arteriosus, small pulmonary artery and aorta muscle cells under hypoxic conditions:an in vitro study

作　　者：洪海筏[1] 陈会文[1] 孙彦隽[1] 刘锦纷[1] 

机构地区：[1]上海交通大学医学院附属上海儿童医学中心心胸外科,上海200127

出　　处：《临床儿科杂志》2007年第10期853-856,共4页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(No.30471712)

摘　　要：目的了解胚胎低氧环境下动脉导管平滑肌细胞(ductus arterious smooth muscle cell,DASMC)、肺小动脉平滑肌细胞(small pulmonary artery smooth musclecell,SPASMC)及相对照的主动脉平滑肌细胞(aorta smooth muscle cell,AOSMC)内钙离子浓度的变化差异。方法培养孕期120d左右胎羊的DASMC、SPASMC和AOSMC。应用特异性的Ca2+荧光指示剂Fluo-3-AM负载细胞,通过激光共聚焦显微镜动态观察模拟胚胎低氧环境下三类细胞游离Ca2+浓度的变化。结果DASMC从正常氧环境转换到低氧环境后3min,细胞内荧光强度(FI)开始降低(P<0.01),并在第8min降至最低点;SPASMC从正常氧环境转换到低氧环境后2min,FI开始升高,并在第9min升至最高点;AOSMC从正常氧环境转换到低氧环境后,其FI浓度保持不变(P>0.05)。结论在胚胎低氧环境下,DASMC胞内游离钙离子浓度降低、SPASMC胞内游离钙离子浓度升高,而AOSMC胞内游离钙离子浓度保持不变。Objectives To determine the changes of intracellular calcium concentration （[Ca^2＋] i） in fetal lamb ductus arteriosus smooth muscle cells （DASMC） , small pulmonary artery smooth muscle cells （SPASMC） and arota smooth muscle cells （AOSMC） under hypoxic conditions. Methods DASMC, SPASMC and AOSMC of fetal lamb at 120-day-old were cultured. Cells were pre-loaded with fluo 3-AM and laser scanning confocal microscopy was used to measure the [Ca^2＋]i of these cells under hypoxic conditions. Results The intracellular fluorescence intensity （FI） of DASMC declined at 3 rain after hypoxia （P 〈 0.01） and reached its lowest level at 8 rain. The intracellular FI of SPASMC increased at 2 rain after hypoxia （P 〈 0.01 ） and reached its peak at 9 min. The intracellular FI of AOSMC did not change significandy throughout the experiment （P 〉 0.05）. Conclusions Under hypoxic conditions, fetal lamb DASMC [Ca^2＋]i decreases while SPASMC [Ca^2＋]i increases and AOSMC [Ca^2＋]i does not change significantly.

关 键 词：动脉导管 肺小动脉 胚胎 低氧 钙离子浓度 

分 类 号：R725.4[医药卫生—儿科]