人类免疫缺陷病毒1型特异性CD8^+T细胞应答中人类白细胞抗原Ⅰ的限制位点  

Study on human leukocyte antigen-[ restriction sites in human immunodeficiency virus type 1 specific CD8^+T cell responses

作　　者：唐漾波[1] 唐小平[1] 金侠 

机构地区：[1]广州市第八人民医院传染病研究所 [2]Infectious Diseases Unit University of Rochester Medical Center,Rochester 14642,USA

出　　处：《中华传染病杂志》2007年第9期534-537,共4页Chinese Journal of Infectious Diseases

基　　金：广州市医药卫生科技重点项目(2005-ZDi-04;2006-ZDi-05);广州市科技局应用基础研究项目(2007J1-C0191);广州市科技局重大科技攻关项目(2006Z1-E0091)

摘　　要：目的了解HIV-1特异性CD8^+T细胞应答及其相关的人类白细胞抗原Ⅰ型(HLA-Ⅰ)的限制位点。方法以位于HIV-1 Gag、Vif、Nef区域的合成肽作为刺激表位,选择来自4例健康者的外周血单个核细胞(PBMC)建立4株EB病毒转化的B淋巴细胞样细胞系(BLCL),以此作为抗原递呈细胞(APC),对1例临床上长期不进展的HIV-1感染者CD8^+T细胞的IFN-γ分泌细胞频率进行体外酶联免疫斑点技术(ELISPOT)测定和HLA-Ⅰ限制位点比对分析。结果在特异性CD8^+T细胞应答中HLA-Ⅰ分子能限制性递呈HIV-1表位,且呈现出高特异性;HLA-Ⅰ位点A03、A26、C04和C08限制性递呈HIV-1 Gag区域表位,A03和C08还限制性递呈Nef区域表位。结论HIV-1 Gag区域表位的HLA-1限制位点可能包括A03、A26、C04和C08,Nef区域表位的限制位点可能包括A03和C08。Objective To investigate human immunodeficiency virus type 1 （HIV-1） specific CD8＋ T cell responses and related human leukocyte antigen Ⅰ （HLA-Ⅰ） restriction sites. Methods Four Epstein-Barr virus （EBV） strains-transformed B-lymphoblastoid cell lines （BLCL） from peripheral blood monocytes （PBMC） of 4 healthy individuals were established. Using synthesized peptides in HIV-1 Gag, Vif and Nef regions as epitopes and using BLCL as antigen-presenting cells （APC）, the frequency of interferon-γ（IFN-γ） secreting cells in CD8＋ T cells from a HIV-infected long term nonprogressor by enzyme-linked immunospot （ELISPOT） assay in vitro were assessed. Moreover, matching analysis of HLA-Ⅰ restriction sites were performed. Results HIV-1 epitopes were presented restrictively by HLA-Ⅰ molecules in specific CD8^＋ T cell responses, and this antigen presenting had high specificity. HLA-Ⅰ restriction sites A03, A26, C04 and C08 contributed to present epitopes in HIV-1 Gag region, A03 and C08 to HIV-1 Nef as well. Conclusion HLA-Ⅰ restriction sites may include A03, A26, C04 and C08 in HIV-1 Gag region, and A03, C08 in Nef region.

关 键 词：HIV-1 CD8阳性T淋巴细胞 HLA抗原 限制位点 表位 酶联免疫斑点技术 

分 类 号：R392[医药卫生—免疫学]