KLIANNTRV是结核抗原Ag85A的HLA-A*0201限制性CTL表位  

Prediction and Identification of HLA-A*0201 restricted CD8^+CTL epitopes in Mycobacterium tuberculosis Ag85A

作　　者：陈燕[1] 娄加陶[1] 吴传勇[1] 周晔[1] 陈波[1] 蒋天舒[1] 仲人前[1] 邓安梅[1] 

机构地区：[1]第二军医大学附属长征医院实验诊断科全军临床免疫中心,上海200003

出　　处：《现代检验医学杂志》2007年第5期1-3,共3页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金(30471616);上海市重大科技攻关基金(04DZ19116);上海市重点科研支撑条件项目(051409012);上海市青年科技启明星计划(QMX01423);上海市重点基础研究项目(05JC14052)资助

摘　　要：目的鉴定结核杆菌抗原Ag85A的HLA-A*0201限制性CTL优势表位。方法采用数据库SYFPEITHI初步预测结核抗原Ag85A的HLA-A*0201限制性CTL表位;经流式细胞术分析抗原肽与HLA-A*0201的亲和力;经时间荧光分辨法检测患者外周血单个核细胞(PBMCs)对抗原肽的增殖反应;最后通过细胞毒实验逐步鉴定Ag85A的HLA-A*0201限制性CTL预测的表位。结果实验证明,预测的位于Ag85A氨基酸序列(242-250aa)的肽表位与HLA-A*0201具有较高的亲和力。结论筛选出的KLIANNTRV(242-250aa)是结核杆菌抗原Ag85A的HLA-A*0201限制性CTL优势表位。Objective To identify HLA-A ＊ 0201 restricted CD8^＋ CTL epitopes in Mycobacterium tuberculosis antigen Ag85A. Methods Firstly,database SYFPEITHI was applied to predict HLA-A ＊ 0201 restricted CD8^＋CTL epitopes in Mycobacterium tuberculosis antigen Ag85A. Secondly,affinity and stability of the predicted peptides with T2 ceil were assayed by FACS. Thirdly,cells proliferation and cytotoxicity induced by peptides were investigated by DELFIA cell proliferation assay and DELFIA EUTDA cytotoxicity test respectively. Results Peptide 3 （242-250aa）had high affinity with HLA-A ＊ 0201 molecules,stimulated PBMCs from PPD＋ healthy donors to proliferate and induce antigen specific CTLs. Only pep3 can give a postitive proliferation response and specific cytotoxicity. Conclusion Pep 3 （KLIANNTRV 242- 250aa） is HLA-A ＊ 0201 restricted CDS＋CTL epitope of Mtb antigen Ag85A,which could be the candidate for TB peptidebased vaccine.

关 键 词：结核杆菌 AG85A CTL表位 预测 鉴定 

分 类 号：R378.911[医药卫生—病原生物学] R392.1[医药卫生—基础医学]