苏太仔猪FUT1基因M307位点多态性与F18大肠杆菌抗病相关性的体外鉴定  

Polymorphisms of the FUT1 gene M307 locus in post-weaning Sutai breed piglet and resistance to F18 fimbrial Escherichia coli in vitro

作　　者：吴圣龙[1] 原志伟[1] 鞠慧萍[2] 黄雪根 华金弟 沈家林 周冠月 王建业[1] 谢恺舟[2] 陈国宏[2] 朱国强[1] 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]扬州大学动物科学与技术学院,江苏扬州225009 [3]苏州市苏太猪育种中心,江苏苏州215000

出　　处：《中国预防兽医学报》2007年第10期783-787,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：江苏省六大人才高峰项目(2006);江苏省高技术(农业)项目(BG2006302)

摘　　要：采用PCR-RFLP方法检测了江苏苏太断奶仔猪FUT1基因M307位点等位基因多态性分布,在所检的49头仔猪中,GG基因型个体16头,AG基因型19头,AA基因型14头。在此基础上,制备上述不同基因型个体仔猪小肠上皮细胞,分别与表达F18ab菌毛的野生型大肠杆菌、表达F18ac菌毛含fed操纵子全基因的重组大肠杆菌和V型系统表面分泌表达F18abFedF亚单位的重组大肠杆菌进行体外黏附试验和黏附抑制试验。研究结果表明:FUT1基因M307位点中GG型和AG型仔猪小肠上皮细胞均能黏附上述3种大肠杆菌,而AA型个体小肠上皮细胞则不能黏附。将上述3种大肠杆菌分别与抗F18ab菌毛高免血清、F18ac菌毛高免血清及抗F18abFedF亚单位单因子血清作用后,则失去黏附仔猪肠上皮细胞能力。上述结果对苏太猪从体外试验上证明了FUT1基因M307位点多态性与断奶仔猪腹泻和水肿病存在着直接的相关性。The polymorphisms of FUT1 gene M307 locus in 49 post-weaning Sutai breed piglets were screened by PCR-RFLP, and three genotype were identified as FUT1 M307^GG （16）, M307^AG （19） and M307^AA （14）. Small intestinal epithelium cells of different genotypes were selected to test the adhesion capability of the wild type E.coli expressing F 18ab fimbriae, the recombinant Escherichia coli expressing FlSac fimbriae or the recombinant Escherichia coli secreting and surface-displaying the FedF subunit of FlSab fimbriae, respectively. The results showed that all of wild type and recombinant Eschenchia coil were able to adhere to the post-weaning small intestinal epithelium cells of both M307^GG and M307^AG genotypes. The adhesion capability could be inhibited after incubation bacterium with the corresponding anti-serium. There appeared to be a direct relationship between polymorphisms of FUT1 gene M307 locus in post-weaning Sutai breed piglets and the resistance to pathogenic F18 fimbrial E.coli, which could be determined by adhesion and adhesion inhibition assays in vitro. The M307^GG and M307^AG genotypes are related to the susceptibility of the PWD and ED, while M307^AA related to the resistance.

关 键 词：仔猪 F18大肠杆菌 受体 FUT1基因 基因多态性 

分 类 号：S852.61[农业科学—基础兽医学]