抗猪附红细胞体单克隆抗体的制备  

Development of monoclonal antibodies against Eperythrozoon suis

作　　者：张浩[1] 吴晓东[2] 张守富 单虎[1] 吴延功[2] 王志亮[2] 刘佩兰[2] 朱绍辉[1] 

机构地区：[1]莱阳农学院动物科技学院,山东青岛266109 [2]中国动物卫生与流行病学中心,山东青岛266032 [3]青岛中仁药业有限公司,山东青岛266329

出　　处：《中国预防兽医学报》2007年第10期814-816,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家"十五"科技攻关项目(2004BA514A)资助

摘　　要：以纯化的猪附红细胞体免疫BALB/c小鼠,运用淋巴细胞杂交瘤技术进行细胞融合,并用间接ELISA方法进行筛选,经过间接ELISA方法、免疫印迹和间接免疫荧光试验进行鉴定,共获得5株分泌抗猪附红细胞体单克隆抗体的杂交瘤细胞,分别命名为1H1、1H2、3A5、5B1和7E11,其单抗亚类鉴定分别属于IgG2b、IgG1、IgG2b、IgG2b和IgG2b。这5株McAb均能与猪附红细胞体全菌蛋白发生特异性反应,而不与猪肺炎支原体、猪链球菌、大肠杆菌和猪繁殖与呼吸综合征病毒发生反应,并且1H1、3A5和5B1能识别同一抗原位点,1H2和7E11识别另一抗原位点。Monoclonal antibodies （McAb） were prepared using spleen cells of BALB/c mice immunized with purified Eperythrozoon suis according to standard hybridoma protocols. Five hybridoma cell lines secreting McAbs against E.suis were obtained following screening by indirect enzyme-linked immunosorbent assay （ELISA） and indirect immunofluorescence assay. These five McAbs, designated as 1H1, 1H2, 3A5, 5B1 and 7E11 respectively, were highly specific for E.suis and didn＇t react with Mycoplasma hyopneumoniae, Streptococcus suis, E.coli, PRRSV. McAbs 1H1, 3A5 and 5B1 were shown against the same epitope while 1H2 and 7E11 against another.

关 键 词：杂交瘤 猪附红细胞体 单克隆抗体 间接ELISA 

分 类 号：S852.43[农业科学—基础兽医学]