丹参对体外培养之晶状体上皮细胞的保护作用  

Protection of Tan-shen to Lens Epithelial Cells in Vitor

作　　者：崔琳[1] 高维奇[1] 刘平[1] 张红[1] 于莉[1] 关立南[1] 

机构地区：[1]哈尔滨医科大学第一附属医院眼科医院眼科三病房,黑龙江哈尔滨150001

出　　处：《中国伤残医学》2007年第5期17-19,共3页Chinese Journal of Trauma and Disability Medicine

摘　　要：目的:研究丹参对体外培养的兔晶状体氧化损伤的保护作用。方法:应用单细胞凝胶电泳法分别测定了0.1mg/ml、0.5mg/ml、1mg/ml浓度丹参液对体外培养72h后晶状体上皮细胞氧化损伤的保护作用,并测定晶状体中SOD、MDA含量。结果:各浓度丹参培养组相对于H2O2培养组晶状体的氧化损伤均有不同程度减轻,1mg/ml丹参剂量组的保护作用更为明显,其尾长、彗星细胞百分率及SOD、MDA含量与对照组相比无显著差异(P>0.05),对照组及各处理组与H2O2培养组相比各指标均有显著差异(P<0.001)。结论:丹参中的有效成分对体外培养的晶状体氧化损伤有明显的保护作用,且呈现剂量依赖的量效关系。Objective：To investigate the protection of Tan-shen on H2O2-induced lens damage of rabbit. Method： The protection of 0.1mg/ml, 0.5mg/ml, lmg/ml Tan-shen on H2Oa-induced lens epithelial cells damage was detected with single cell gel electrophoresis assay at 72 hours after culture in vitor ,and detected the contents of SOD, MDA in lens. Results：The various concentration groups of Tan-shen were significantly increased campared H2O2-induced group, 0.8mg/ml group have an advantage on other groups.There was not significant differece with control groups in tail DNA%, tail length and the contents of SOD, MDA（P〉0.05）, there was great significant differece at treatment groups and damage groups in various indexs（P〈0.001）. Conclusion： Tan-shen could protect lens epithelial cells against oxidative stress. Its effect might be dose-dependent.

关 键 词：丹参 晶状体 氧化损伤 体外培养 

分 类 号：R965[医药卫生—药理学]