终末糖基化终产物对糖尿病大鼠甲状腺组织的影响  

The Effects of Advanced Glycation End Products on the Thyroid Tissues in Diabetic Rats

作　　者：张宏[1] 董如娇[1] 赵伟[1] 郑凝[1] 常宝成[1] 王家驰[1] 方佩华[2] 

机构地区：[1]天津医科大学代谢病医院,300070 [2]天津医科大学总医院核医学科

出　　处：《天津医药》2007年第10期758-760,F0003,共4页Tianjin Medical Journal

基　　金：天津市科委资助项目(项目编号:06YFJMJC07800)

摘　　要：目的:探讨终末糖基化终产物(AGEs)对甲状腺组织的影响及其机制。方法:链脲佐菌素制备糖尿病(DM)大鼠模型(DM组),并设对照组,每组25只,应用免疫组织化学和Western Blotting方法检测大鼠甲状腺组织中AGEs和甲状腺球蛋白(TG)、甲状腺过氧化物酶(TPO)的生成情况。结果:HE染色示DM组较对照组甲状腺滤泡上皮细胞变扁平,胶质中吸收空泡减少;免疫组织化学染色示对照组和DM组甲状腺组织中AGEs和TG、TPO均为阳性,AGEs阳性部位主要在间质,TG、TPO阳性部位主要在胶质和胞浆;Western Blotting示DM组较对照组甲状腺组织AGEs生成明显增多,TG和TPO的含量降低,差异均有统计学意义(P<0.01)。结论:甲状腺组织是高血糖攻击的靶器官之一,高血糖对甲状腺组织的损害表现为功能减低。Objective： To study the effects and mechanisms of advanced glycation end products （AGEs） of the thyroid tissues in diabetic rats. Methods： The rat model of diabetes was induced with streptozocin. The values of AGEs, thyroglobulin （TG） and thyroid peroxidase （TPO） of the thyroid tissues were detected by immunohistoehemistry and Western blotting methods. Results： Under microscope, the thyroid follicle cell appeared applanatus and absorbed vaeuolus was decreased in diabetic rats. The positive expressions of AGEs, TG and TPO were found by immunohistoehemistry in diabetic and control rats. The positive expression of AGEs presented yellow in periplast, and positive expression of TG and TPO located in endoehylema and colloid. The expression of AGEs was significant increased in diabetic group compared to control group （P 〈 0.01）,while the levels of TG and TPO were significant decreased in diabetic group （P 〈 0.01）. Conclusion： The thyroid tissues may be one of the target organs of hyperglycemia lesion. The hyperglycemia lesion of thyroid tissues represents lower function.

关 键 词：糖尿病 实验性 糖基化终产物 高级 甲状腺 大鼠 SPRAGUE-DAWLEY 疾病模型 动物 

分 类 号：R581.1[医药卫生—内分泌]