孕激素对在位内膜构建子宫内膜异位症裸鼠的影响  

Effects of Progesterone on Ectopic Tissue Formation in Nude Mice

作　　者：张秀兰[1] 李妍涵[2] 糜若然[1] 

机构地区：[1]天津医科大学总医院妇产科,300052 [2]中国医学科学院血液病研究所

出　　处：《天津医药》2007年第10期766-768,801,共4页Tianjin Medical Journal

摘　　要：目的:比较雌激素(E组)与雌孕激素(E+P组)两种体外培养条件对在位内膜构建裸鼠子宫内膜异位症(EMs)模型的影响。方法:将EMs患者的在位内膜碎片加入到含10%小牛血清的去酚红DMEM/Ham’sF-12培养液中,同时E组加入雌激素(10nmol/L),E+P组加入孕激素(100nmol/L)及雌激素(10nmol/L)分别培养24h后接种于裸鼠腹腔。结果:E+P组种植成功率高于E组,与E组比较病灶数目有增多趋势(Z=2.21,P=0.05),病灶直径减小(Z=2.24,P<0.05)。免疫组化显示E+P组培养后在位内膜腺体的表达无显著性改变,但PR、Ki-67表达有减弱趋势;成模的裸鼠异位灶腺体中Ki-67的表达也降低(Z=2.27,P<0.05),PR有降低趋势。异位灶间质中微血管密度显著减低(Z=2.73,P<0.05)。雌孕激素培养后获得的异位灶间质细胞增生,异位灶与周围组织接合更为紧密。结论:加入孕激素的体外培养条件不影响在位内膜成模,但血管生成、细胞增殖并未加强。Objective： To investigate the effect of progesterone on DMEM/Ham＇s F-12 solution, which contained estrogen, and to assess the functions of progesterone in ectopic tissue formation in nude mice. Methods： The eutopic tissues collected from patients with endometriosis（4 cases）or adenomyosis（ 1 case）were cultured 24 hours in vitro with estrogen （10 nmol/L,E group）or estrogen plus progesterone （100 nmol/L,E＋P group） based on DMEM/Ham＇s F-12 solution, respectively. After hatching, the tissues were transplanted intraperitoneaUy into the nude mice. The expression of VEGF, Ki-67 and PR were examined by immunohistochemical analysis before and after 24 h culture in vitro to find the different hormone affection in the two cultural conditions. Results： There was a tendency to increase in number of focus （Z = 2.21, P 〈 0.05 ）and decrease in diameter of focus（Z = 2.24, P 〈 0.05 ）in E＋P group compared with those of E group. There was a tendency to decrease in expression of PR and Ki-67 in E＋P group compared with those of E group. The expression of Ki-67 appeared decreasing in focus of mouse modal （Z = 2.27 ,P 〈 0.05 ）. The microvessel density was significantly decreased in focus （Z = 2.73,P 〈 0.05）. Conclusion： Progesterone （100 nmol/L）cuhural condition in vitro may be conducive to forming the ectopic tissue in nude mice but no enhancing for micro blood vessel formation and cytoplasm.

关 键 词：子宫内膜异位症 孕激素类 雌激素类 血管内皮生长因子类 细胞增殖 小鼠 裸 

分 类 号：R711.71[医药卫生—妇产科学]