三种鞘翅目昆虫的脑和咽下神经节的神经解剖学及5-HT阳性反应模式研究(英文)  

NEUROANATOMY AND SEROTONIN IMMUNOREACTIVITY IN THE BRAIN AND SUBOESOPHAGEAL GANGLION OF THREE BEETLES

作　　者：胡西旵[1] 田喜梅[2] 瞿玉堂[1] 赵志富[1] 刘天依[1] 宋传涛[3] 暴学祥[1] 

机构地区：[1]东北师范大学生命科学学院昆虫脑神经生物学实验室,长春130024 [2]吉林大学地面机械仿生技术教育部重点实验室,长春130022 [3]东北师范大学城市与环境科学学院,长春130024

出　　处：《神经解剖学杂志》2007年第5期451-461,共11页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(No. 30470546)资助项目

摘　　要：采用树脂石蜡(CP)组织包埋连续切片技术,结合免疫组化链霉菌抗生物素蛋白-过氧化物酶(SP)染色法,首次对三种甲虫(榆紫叶甲、小青花金龟、马铃薯瓢虫)的脑进行了详细观察研究,分析了三种甲虫脑组织结构特征,初步构建了三种甲虫脑内5-HT抗原系统免疫组化反应模式。结果显示,分类地位相近的三种甲虫脑结构大小相差悬殊:小青花金龟脑结构相对发达,蕈形体结构显著,具有发达的冠和叶,嗅叶发达;马铃薯瓢虫和榆紫叶甲的冠极度退化,嗅叶相对不发达;但榆紫叶甲具有十分发达的网膜后纤维和围咽神经。5-HT阳性纤维在三种甲虫的所有神经纤维网中几乎都有分布,且反应模式相似,但各神经纤维网特别是神经节层染色强度差异显著。5-HT阳性胞体有成群分布现象,且在三种甲虫脑中的空间分布位置基本一致。结果表明,三种甲虫可能由于长期生活习性的不同,在进化压力作用下产生了适应辐射,榆紫叶甲的分类地位可能相对较为低等。不同昆虫脑内5-HT阳性反应产物分布模式的相似性表明,5-HT在不同昆虫中极有可能发挥着相似的生理功能,而且5-HT可能参与调节饮食行为及视觉系统的敏感度。The structure and 5-HT immunoreactivity in the brain and suboesophageal ganglion of three beetles, Ambrostoma quadriimpres- sum, Henosepilachna vigintioctomaculata and Oxyeetonia jucunda, were first studied by means of colophony-paraffin embedding serial sec- tion technique and strepteavidin-peroxidase immunohistochemical method. The results showed that the brains of these three taxonomically closely related beetles were remarkably different in composition and size. Mushroom bodies and antennal lobes in Oxycetonia jucunda were conspicuous. Calyces and lobes of the mushroom bodies were much developed. In contrast, calyces of Ambrostoma quadriimpressum and Henosepilachna vigintioctomaculata were extremely undeveloped. However, the postretinal fibres and circumpharyngeal nerves of Ambrostoma quadriimpressum were highly developed. In the three beetles, 5-HT immunoreactivity was present in all neuropils of the brain and the suboesophageal ganglion. The pattern of 5-HT immunoreactivity and the localization of immunoreactive somata which often clustered into groups were similar among these beetles, while the immunoreactivity intensity was distinct, especially in the lamina. The results suggest that the three beetles have given rise to adaptive radiation under the evolutionary pressure because of the long-term different life styles and living environments in which the taxonomic status of Ambrostoma quadriimpressum is relatively low. The similarity of the pattern of 5-HT immunoreactivity and localization of some positive somata among the three beetles raise the possibility that 5-HT seemes to serve similar physiological function in different insects. Furthermore, 5-HT might be involved in modulating the ingestion by regulating muscular activity and visual sensitivity.

关 键 词：5-HT 免疫组织化学 甲虫 脑 咽下神经节 

分 类 号：Q96[生物学—昆虫学]