离体的细胞外电刺激装置的优化和验证(英文)  

OPTIMISATION AND VALIDATION OF AN IN VITRO EXTRACELLULAR ELECTROSTIMULATING DEVICE

作　　者：夏蓉[1] Franis Berger Alim-Louis Benabid 

机构地区：[1]上海交通大学医学院解剖学教研室,上海200025 [2]法国国立健康和医学研究所

出　　处：《神经解剖学杂志》2007年第5期489-494,共6页Chinese Journal of Neuroanatomy

基　　金：法国国立健康和医学研究所,美国Medtronic公司赞助

摘　　要：为了研究高频电刺激的机理,我们设计了一个离体的细胞外电刺激培养细胞的装置,它能适用临床上外科治疗巴金森病的深部脑刺激的参数。此装置在24孔培养板相邻的两个孔中,架上钛金属丝桥;或者在10 cm直径的培养皿中,安置2条平行的钛金属丝。我们证明它在GH3和PC12细胞的培养中无毒性。初步的实验结果显示它适用于离体细胞新陈代谢变化的实验,基因芯片及蛋白芯片等技术的实验。In order to study the mechanisms of high frequency stimulation （ HFS）, we designed an in vitro device to deliver extracellular electrical stimulation to live cells in culture, using the same parameters as in the surgical treatment of Parkinson＇s disease （PD）. The system was made of a 24 wells culture plate electrified with titanium wire bridges or two titanium wires placed parallel in a culture dish of 10 centimetres diameter. The extremities of the series of wells were connected to a constant current generator. The result showed that this system had no toxicity on GH3 and PC12 cell cultures. Preliminary experiences demonstrate that this system is suitable for the study with DNA and protein chip technologies.

关 键 词：巴金森病 高频电刺激 细胞培养 新陈代谢 

分 类 号：Q42-3[生物学—神经生物学]