NGF和GM_1联合应用对去细胞异种神经支架移植后神经再生和修复的影响  

EFFECTS OF CO-APPLICATION OF NGF AND GM_1 ON THE NERVE REGENERATION AND RECOVERY AFTER XENOGENEIC ACELLULAR NERVE SCAFFOLD TRANSPLANTATION IN THE RAT

作　　者：焦旭文[1] 何仲义[1] 李军平[1] 韩怀钦[1] 王效军[1] 马江波[1] 

机构地区：[1]宁夏医学院人体解剖学教研室,银川750004

出　　处：《神经解剖学杂志》2007年第5期495-500,共6页Chinese Journal of Neuroanatomy

基　　金：教育部科学技术研究(重点)项目(No204153);宁夏教育厅科研基金(NoJY2002124)资助项目

摘　　要：为了探讨神经生长因子(NGF)和单唾液酸四己糖神经节苷脂(GM1)联合应用对去细胞异种神经支架移植后神经再生及功能恢复的影响,本研究将SD大鼠随机分为生理盐水对照组、NGF治疗组、GM1治疗组、NGF+GM1联合治疗组,选取兔胫神经进行化学萃取,形成去细胞异种神经支架桥接大鼠10mm坐骨神经缺损,移植前分别用等渗盐水(NS)、NGF液、GM1液或NGF+GM1液浸泡去细胞异种神经支架,术后各组大鼠术侧小腿肌内分别注射NS液、NGF液、GM1液或NGF+GM1液。术后4、8周行大体观察并用神经电生理、肌湿重、免疫组织化学等方法测定神经纤维再生及功能恢复情况。结果显示:在同一时间点,NGF+GM1联合治疗组坐骨神经运动传导速度恢复率、小腿腓肠肌复合动作电位波幅恢复率、小腿三头肌湿重恢复率均优于单独用药组(P<0.05);免疫组织化学结果显示NGF+GM1联合治疗组有大量再生有髓神经纤维顺畅地通过远端吻合口。本研究结果提示联合应用NGF和GM1可明显促进去细胞异种神经支架移植后的神经纤维再生与功能恢复。To explore the effect of co-application of the nerve growth factor （NGF） and monosialotetranexosyl gangliosides （ GMl ） on nerve regeneration and function recovery after xenogeneic acellular nerve scaffold transplantation, SD rats were randomly divided into four groups： normal saline （NS） control, NGF-treated, GM1-treated and NGF ＋ GMl-treated group. The rabbit tibial nerves were sectioned to take chemical extraction for making the scaffold. The sciatic nerve defects were bridge-connected with 10 mm long xenogeneic acellular nerve scaffold. Before transplantation, the xenogeneie acellular nerve scaffolds were dipped into NS, NGF, GMl and NGF ＋ GMl , respectively. After operation, the rats were injected with NS, NGF, GMl and NGF ＋ GM1 , respectively, into muscles of the lower limb on the surgical side. In each group, after the 4th and the 8th week of the operation a series of examinations were carried out, such as nerve electrophysiological, wet weight of the muscle and immunohistochemical staining, to investigate the nerve regeneration and functional recovery. It was demonstrated that, in the same time span, the results of NGF ＋ GM1 treated group were significantly better than those of the solely treated group （P 〈 0.05 ） in the following measurements： recovery rate of the motor nerve conduction velocities （MNCV%）, triceps surae recovery rate of the complex muscular action potential （ CMAP% ） and the recovery rate of wet weight of triceps surae. The results of the immunohistochemical staining showed that there were a large amount of regenerated and mynelated nerve fibers in the scaffold and they went through the distal stoma into the scaffold smoothly. The present results suggest that coapplication of NGF and GM1 can obviously enhance the peripheral nerve regeneration and function recovery in xenogeneic acellular nerve scaffold graft.

关 键 词：神经生长因子 单唾液酸四己糖神经节苷脂 去细胞异种神经支架 异种神经移植 神经再生 

分 类 号：R651.3[医药卫生—外科学]