广西眼镜蛇毒神经生长因子对颅脑创伤大鼠细胞免疫功能的影响  

EFFECT OF GUANGXI COBRA VENOM NERVE GROWTH FACTOR ON CELLULAR IMMUNE FUNCTIONS IN BRAIN TRAUMA RATS

作　　者：张学荣[1] 罗小玲[2] 宋慧[1] 韦敏[1] 舒雨雁[1] 

机构地区：[1]广西医科大学医学科学实验中心蛇毒研究所,南宁530021 [2]广西医科大学附属肿瘤医院

出　　处：《广西医科大学学报》2007年第4期536-538,共3页Journal of Guangxi Medical University

基　　金：广西自然科学基金资助课题(No.9811039;桂科能0443001-17)

摘　　要：目的:探讨广西眼镜蛇毒神经生长因子(nerve growth factor,NGF)对颅脑创伤大鼠免疫功能的影响。方法:实验SD大鼠36只,随机分为3组,分别为颅脑创伤模型治疗组(NGF组)、颅脑创伤模型对照组、正常对照组。采用ELISA法检测治疗后各组大鼠脾细胞培养液中IL-2水平;MTT法检测脾细胞的增殖能力和鸡红细胞吞噬百分率及吞噬指数评价大鼠腹腔巨噬细胞吞噬活力;细胞组化染色法检测外周血醋酸奈酸脂酶(ANAE)阳性细胞变化。结果:颅脑创伤模型对照组大鼠脾细胞体外增殖能力、脾细胞分泌IL-2能力、ANAE阳性细胞百分比和腹腔巨噬细胞吞噬活力显著低于正常对照组(P<0.01或P<0.05);而采用NGF治疗的颅脑创伤模型治疗组大鼠的脾细胞体外增殖能力、脾细胞分泌IL-2能力和ANAE阳性细胞百分比、腹腔巨噬细胞吞噬活力均比颅脑创伤模型对照组显著升高(P<0.01或P<0.05),与正常对照组比较差异无统计学意义(P>0.05)。结论:NGF治疗能提高颅脑创伤大鼠的细胞免疫功能。Objective： To investigate the effect of Guangxi Cobra Venom nerve growth factor on cellular immune functions in brain trauma rats. Methods： 36 experiment SD rats were randomly divided into three groups： brain trauma care group（treated with NGF）; brain trauma control group and normal control group. The proliferation of the splenocyte was detected by MTT method, and the level of IL-2 by ELISA assay. The phagocytotic activity of rats peritoneal macrophages was determined by the chicken-red blood cell phagocytosis rate and phagocytosis index, and change of ANAE masculine cell in peripheral blood was observed by cell immunohistochemistry method Results： The reproductive activity of splenocyte in vitro, the production of IL-2 of splenocyte,the phagocy- totic activity and percentage of ANAE masculine cell of brain trauma control group were obviously lower than those of the normal control（ P d0.01 or P d0.05）. However, in the NGF cure group, the reproductive activity of splenocyte in vitro, the production of IL-2 of spleen T lymphocytes, the phagocytotic activity and percentage of ANAE masculine cell increased strikingly,and were higher than those of the brain trauma in the control group（ P 〈0.01 or P 〈0.05）and similar to the normal control（ P 〉0.05）. Conclusion： NGF therapy can improve the cellular immune functions of the brain trauma rats.

关 键 词：神经生长因子 大鼠 脑创伤 免疫功能 

分 类 号：R642[医药卫生—外科学]