HSPCO11基因在人毛乳头细胞表达的生物学功能  

Biologic function of HSPCOll gene expressing in human dermal papilla cells of hair follicle

作　　者：杨卫兵[1] 郝飞[1] 杨希川[1] 宋志强[1] 杨斌[1] 王继文[1] 

机构地区：[1]第三军医大学西南医院皮肤科,重庆400038

出　　处：《中华医学美学美容杂志》2007年第5期284-287,共4页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：国家自然科学基金资助项目（编号：30300180）

摘　　要：目的 探讨HSPC011基因在人毛乳头细胞表达的生物学功能。方法 应用Nucleo-lector^TM基因转染技术，分别将质粒pCI-neo、绿色荧光蛋白质粒pEGFP-N1、真核表达重组质粒pCI-neo／HSPC011转染人毛乳头细胞和3T3细胞，了解HSPC011基因表达对这些细胞生长特性的影响。结果 通过观察绿色荧光蛋白在人毛乳头细胞和3T3细胞的表达，发现Nucleofector^TM基因转染效率分别约为70％和30％。HSPC011基因在人毛乳头细胞表达后，细胞形态幼稚而类似于3T3细胞，但其凝集性生长特性保持不变；HSPC011基因表达对毛乳头细胞的增殖活性无明显影响。HSPC011基因转染3T3细胞后，对3T3细胞的生长特性无任何影响。结论 初步体外实验表明，HSPC011基因在毛乳头细胞表达可能具有调节细胞分化的作用。Objective To study the biologic function of hematopoietic stem/progenitor cell （HSPC） 011 gene, which was found to specifically express in the dermal papilla cells of hair follicle. Methods The plasmid pCI-neo, the pEGFP-N1 （green fluorescent protein plasmid） and the recombi- nant eukaryotic expressing plasmid pCI-neo/HSPC011 were differently transfected into the human dermal papilla cells and 3T3 cells by NucleofectorTM technique, then the effects of the expression of HSPC011 gene in these cells were investigated. Results Based on the observation of the expression of the green fluorescent protein in the human dermal papilla cells and 3T3 cells, the gene transfecting efficiency with NucleofectorTM technique was about 70% and 30%, respectively. While the HSPC011 gene expressed in the dermal papilla cells, which were immature and were similar to 3T3 cells in shape, but their aggregative behavior reserved. The activity of proliferation of human dermal papilla cells was not changed by the expression of HSPC011 gene. As for the expression of gene HSPC011, there were no effects on the biologic functions of the 3T3 cells. Conclusion The expression of HSPC011 gene might affect the differentiation of the dermal papilla cells in vitro.

关 键 词：毛囊 毛乳头细胞 基因 HSPC011基因 生物学功能 

分 类 号：R3[医药卫生—基础医学]