激光对小鼠皮肤碱性成纤维细胞生长因子和β1转化生长因子表达的影响  

Effects of 1 320 nm laser irradiation on expression of bFGF and TGF-β1 in mouse skin

作　　者：郑振汶[1] 许爱娥[1] 郑俊惠[1] 金宪强[1] 沈静[1] 郑涵[1] 程浩[2] 

机构地区：[1]杭州市第三人民医院皮肤科,杭州310009 [2]浙江大学医学院附属邵逸夫医院

出　　处：《中华医学美学美容杂志》2007年第5期292-294,共3页Chinese Journal of Medical Aesthetics and Cosmetology

基　　金：杭州市科技局基金资助项目（编号：20070433Q18）

摘　　要：目的 探讨1320nm波长非剥脱性激光对小鼠皮肤碱性成纤维细胞生长因子（bFGF）、β1转化生长因子（TGF-β1）表达的影响，为非剥脱性激光嫩肤提供实验依据。方法 以昆明小鼠为动物模型，共18只，分为A、B、C3组，每组6只。A组激光照射1次，B组激光照射2次，间隔1min，C组激光照射3次，间隔1min。共4个疗程，每个疗程间隔1周。选择1320nm波长激光，20J／cm^2能量照射小鼠背左侧皮肤，以背右侧作自身对照。用免疫组化法检测bFGF和TGF-β1在受照射小鼠皮肤真皮中的表达。以真皮成纤维细胞胞浆含黄色或棕黄色颗粒为阳性细胞，计算每高倍视野阳性细胞数。结果 1320nm波长激光照射后小鼠皮肤bFGF和TGF-β1表达增加，其中激光照射3次组，每高倍视野阳性细胞数为（1．57±1．12）个，与各组比较，差异有统计学意义（P〈0．01）。结论 小鼠皮肤受1320nm波长激光照射后，其真皮bFGF和TGF-β1表达增加，表达量与激光照射次数有关。Objective To explore the effect of 1320 nm nonablative laser energy on the expression of bFGF and TGF-β1 in mouse skin. and to offer experimental evidence for nonoblative skin rejuvenation. Methods We used the Kunming mice （N= 18） as subjects. The mice were divided into three groups （A, B, C）, six each. Groups A, B, and C were irradiated with 1320nm cool-touch laser （20 J/cm^2） in the skin of left back; Groups B and C were irradiated two and three times, respectively. The skin of right back in all groups was adopted as control. The expression of bFGF and TGF-β1 in the mouse skin was examined by the immunohistochemistry. The cells whose cytoplasm contained yellow or brown-yellow granules were regarded as positive cells. The positive cells were counted in each high power field. Results Expression of bFGF and TGF-β1 in mouse derm increased after 1 320 nm laser irradiating. In the irradiated three times group the count of the positive cells in each high power field was 1. 57± 1. 12. There were significant differences in the expression of bFGF and TGF-β1 between the group irradiated by three times and others （P〈0.01）. Conclusions 1320 nm laser promotes the expression of bFGF and TGF-β1 in the mouse＇s derm. The number of the positive cells is related with the times of irradiation.

关 键 词：非剥脱性嫩肤 激光 免疫组化 碱性成纤维细胞生长因子 Β1转化生长因子 

分 类 号：R644[医药卫生—外科学]