Studies on Prunus Necrotic Ringspot Virus (PNRSV) Occurring on Lily  

作　　者：HAN Li-juan LIU Wen-hong 

机构地区：[1]Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100025, P.R.China [2]Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, P.R.China

出　　处：《Agricultural Sciences in China》2007年第10期1201-1208,共8页中国农业科学（英文版）

摘　　要：This study was to molecular identify Prunus necrotic ringspot virus （PNRSV） occurring on lily. Lily corms were randomly selected and grown in the greenhouse. The total RNAs were extracted from younger leaves, and partial amplification of the CP gene was performed by RT-PCR with primer pairs specific for PNRSV. An expected cDNA fragment of about 450 bp was amplified from lily, cloned into pGEM-T-easy vector, sequenced, and shared 84.5-99.1% homology with the 27 PNRSV isolates reported previously at the nucleotide level, indicating that lily is a new natural host of PNRSV.This study was to molecular identify Prunus necrotic ringspot virus （PNRSV） occurring on lily. Lily corms were randomly selected and grown in the greenhouse. The total RNAs were extracted from younger leaves, and partial amplification of the CP gene was performed by RT-PCR with primer pairs specific for PNRSV. An expected cDNA fragment of about 450 bp was amplified from lily, cloned into pGEM-T-easy vector, sequenced, and shared 84.5-99.1% homology with the 27 PNRSV isolates reported previously at the nucleotide level, indicating that lily is a new natural host of PNRSV.

关 键 词：LILY Prunus necrotic ringspot virus RT-PCR IDENTIFICATION 

分 类 号：S68[农业科学—观赏园艺]