马立克氏病(MD)胚胎免疫攻毒后病毒抗原的定位研究  

STUDIES ON THE DETECTION OF MAREKS DISEASE VIRUS IN EMBRYONALLY VACCINATED CHICKENS

作　　者：鲍恩东[1] 陈万芳[1] 

机构地区：[1]南京农业大学动物医学院

出　　处：《畜牧兽医学报》1997年第3期272-277,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家博士点基金

摘　　要：本试验利用异硫氰酸荧光黄标记抗体和免疫金标记探针，对胚胎免疫攻毒试验鸡组织脏器中马立克氏病病毒（ＭＤＶ）抗原进行定位及动态观察。结果发现，不同组织细胞内ＭＤＶ抗原的表现形态略有不同，淋巴组织中阳性细胞内ＭＤＶ抗原多呈颗粒状或斑点状定位于细胞浆、细胞核内；脏器组织中阳性着染的实质细胞内病毒抗原则主要呈均匀弥散性分布于感染细胞（肾小管上皮细胞、肝细胞）的胞浆中而不发生瘤变。阳性细胞动态观察的结果则显示，胚胎免疫组试验鸡虽然不能阻止ＭＤＶ的感染，但却能明显降低ＭＤ肿瘤的转化率。不过。Detection and dynamic observation of Mareks disease virus (MDV) have been made in organ tissues of embryonally vaccinated chickens, combined with immunofluorescent and immunogold silver stain techniques The results show that different forms of MDV antigens in tissue cells were seen, with particles or dots in the cytoplasm and nucleus of the positive cells in lymph tissues, and with MDV antigens diffusing uniformally in the cytoplasm of the positive cells in visceral tissues Although embryonal vaccination couldnt prevent chickens from MDV infection, but it obviously reduced the transforming rate of MDV infected cells However, no clear law was found for the number and distribution of the positive cells in organ tissues with challenge time

关 键 词：马立克氏病 胚胎免疫 免疫荧光 免疫胶体金 

分 类 号：S855.3[农业科学—临床兽医学] S852.4[农业科学—兽医学]