NM-3含药血清对体外胃癌细胞株SGC-7901生长和VEGF及其受体KDR、Flt-1表达的影响  

Effects of NM-3-containing serum on endothelial growth factor and its receptors KDR and Fit-1 in human gastric cancer SGC-7901 cell line

作　　者：宋明全[1] 朱金水[1] 朱励[1] 张强[1] 李沁[2] 郭花[1] 张卫平[1] 

机构地区：[1]上海交通大学附属第六人民医院消化科,上海市200233 [2]美国休斯顿大学药学院,美国休斯顿77024

出　　处：《世界华人消化杂志》2007年第25期2704-2708,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨NM-3含药血清对胃癌SGC-7901细胞的抑制作用.方法:将SGC-7901胃癌细胞分成空白对照组、血清对照组、NM-3含药血清低、中、高剂量实验组共5组.在不同时间段以流式细胞仪检测细胞的凋亡率,分析细胞周期分布;应用逆转录聚合酶链式反应(RT-PCR)法检测各组胃癌细胞中VEGF及其受体KDR,Flt-1 mRNA的表达.结果:NM-3含药血清3组随着时间的延长凋亡率增高,呈时间剂量依赖性,其高、中剂量组在6h(16.80%±0.40%,25.20%±0.86%)、24h(26.95%±3.25%,43.62%±3.53%)、72 h(39.85%±4.10%,54.35%±5.42%)内的细胞早期凋亡率与空白,血清两个对照组相比有显著性差异(5_34%±0.28%,5.66%±0.72%:6.91%±1.06%,8.90%±0.86%;6.87%±1.24%,8.06%±0.78%;P<0.01).随着NM-3药物剂量的增加,G_0/G_1期细胞增多,而S期及G_2/M期细胞则相应减少.细胞培养72h后,NM-3含药血清高,中,低剂量组与两对照组比较可显著抑制VEGF及其受体KDR,Flt-1 mRNA的表达(P<0.05).在72h后细胞凋亡率,细胞周期以及胃癌细胞中VEGF及其受体KDR,Flt-1 mRNA的表达上,高剂量组与中低剂量组均有明显差异(P<0.05).结论:NM-3含药血清可抑制胃癌组织的血管生长,并可诱导胃癌细胞凋亡.AIM： To study the inhibitory effect of anti- angiogenesis agent NM-3-containing serum on human gastric cancer SGC-7901 cell line.METHODS： Human gastric cancer SGC-7901 cell line was cultured with high, medium and low doses of NM-3-containing serum, and two control groups were cultured with medium alone and medium with serum. Cell cycle distribution and apoptosis rate were determined by flow cytometry. After 72 hours incubation, mRNA for vascular endothelial growth factor （VEGF） and its receptors, including KDR and Fit-1, was detected by reverse-transcriptase polymerase chain reaction （RT-PCR）. RESULTS： The apoptosis rate in the high- （25.20% ± 0.86%, 43.62% ± 3.53%, 54.35% ± 5.42%）and medium-dose （16.80% ± 0.40%, 26.95% ± 3.25%, 39.85% ± 4.10%） groups was significantly increased compared with that in the two control groups were cultured with meduim alone （5.34% ± 0.28%, 6.91% ± 1.06%; 6.87% ± 1.24%） and meduim with serum （5.66% ± 0.72%, 8.90% ± 0.86%, 8.06% ± 0.78%） after 6, 24 and 72 hours culture. The apoptosis rate was increased with prolongation of incubation time and NM-3 dose. The apoptosis rate in the high-dose group was higher than in the medium and low dose groups （P 〈 0.05）. After 72 hours in the high-dose group, the proportion of cells in G0/G1 phase was higher, and the number in S, G2/M phase was lower than that in the medium- and low-dose groups. There were significant differences between the proportion of cells in G0/G1, S and G2/M phase in the high- and medium-dose groups and the two control groups （P 〈 0.01）. mRNA of VEGF and its receptors KDR and Fit-1 decreased in SGC-7901 cells in the high-, medium- and low-dose NM-3 groups after 72 hours. Compared with the two control groups, the high-dose group significantly inhibited mRNA transcription for VEGF and its receptors KDR and Fit-1 （P 〈 0.01）, and the medium- and low-dose groups obviously inhibited mRNA transcription （P 〈 0.05）. CONCLUSION： NM-3-containing serum can inhibit vascul

关 键 词：胃癌 癌细胞株 NM-3 血管内皮生长因子 KDR FLT-1 逆转录聚合酶链式反应 

分 类 号：R735.2[医药卫生—肿瘤]