依那普利拉对严重烫伤大鼠早期心肌损害的防治作用  

Protective effects of administration of enalaprol maleate on rat myocardial damage in early stage of burns

作　　者：张兵钱[1] 黄跃生[1] 张家平[1] 张东霞[1] 党永明[1] 王广[1] 胡炯宇[1] 雷泽源[1] 肖荣[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,创伤,烧伤与复合伤国家重点实验室,重庆400038

出　　处：《中华烧伤杂志》2007年第5期335-338,共4页Chinese Journal of Burns

基　　金：国家重点基础研究发展规划(2005CB522601);全军医学科学技术研究"十一五"计划专项课题(06Z033)

摘　　要：目的了解依那普利拉对烧伤后早期心肌损害的防治效果。方法将60只SD大鼠制成30%TBSAⅢ度烫伤模型,分为单纯烫伤组(30只,伤后常规补液)和依那普利拉组(30只,伤后立即一次性腹腔注射依那普利拉1 mg/kg并行常规补液)。另取6只大鼠不致伤作为正常对照组。检测正常对照组及2组烫伤大鼠伤后1、3、6、12、24 h血清心肌肌钙蛋白I(cTnI)的含量、心肌型肌酸激酶同工酶(CK-MB)的活性,并观察心肌组织病理学变化。结果(1)单纯烫伤组大鼠伤后各时相点cTnI、CK-MB均显著高于正常对照组(P<0.01)。依那普利拉组伤后各时相点cTnI为(1.32±0.12)～(2.47±0.22)μg/L,均显著低于单纯烫伤组[(6.42±0.96)～(15.10±3.69)μg/L,P<0.01];其各时相点CK-MB活性为(438±68)～(5569±322)U/L,亦均低于单纯烫伤组[(2556±74)～(8047±574)U/L,P<0.05或P<0.01]。(2)与正常对照组比较,单纯烫伤组伤后出现心肌细胞浊肿、间质血管扩张充血、炎性细胞浸润等病理改变;依那普利拉组病变程度较之减轻。结论大鼠严重烫伤后早期心肌组织受损明显,依那普利拉能显著减轻这些损害。Objective To investigate the preventive and therapeutic effects of enalapril maleate（ Enalaprilate） （E） on myocardial damage in early stage after burns. Methods A total of 60 SD rats were sub- jected to 30 % TBSA Ⅲ degree scald injury, and randomly divided into scald group （ with conventional fluid transfusion after scald） and ENA group （with intraperitoneal injection of lmg/kg Enalaprilat after scald ）. Normal control consisted of 6 rats. Plasma levels of cTnl and CK-MB were determined in all the groups at 1, 3,6,12,24 post-scald hours （PSH） by enzyme linked immunosorbent assay. The pathological changes in myocardium were observed at the same time-points. Results （ 1 ） The serum level of cTnl and CK-MB in scald group were significantly higher than that of normal controls at each time-point（ P 〈 0.01 ）. The serum level of cTnl and CK-MB in ENA group were（ 1.32 ±0.12 μg/L to 2.47 ±0.22μg/L） and （438 ±68 U/L to 5569±322 U/L） , respectively, which were obviously lower than those in B group（6.42 ±0.96 μg/L to 15.10 ± 3.69 μg/L） and （2556 ± 74 U/L to 8047± 574 U/L, P 〈 0.05 or P 〈 0.01 ） at different timepoints. （2） Compared with normal controls, cloudy swelling, stromal blood vessel dilatation and congestion , imflammatory cell infiltration were observed in scald group, but these pathological changes were less marked in ENA group. Conclusion Severe myocardial damage in rat occurred early after burns. Enalaprilat injection can markedly alleviate myocardial damage.

关 键 词：烧伤 心肌 依那普利拉 

分 类 号：R96[医药卫生—药理学]