透明颤菌血红蛋白基因在提高枯草芽孢杆菌产脂肽功能中的研究  

Studies on the Function of Vitreoscilla Hemoglobin Gene in Improving Surfactin Producing Rate of Bacillus subtilis

作　　者：王大威[1] 胡鸢雷[2] 刘永建[1] 林忠平[2] 

机构地区：[1]大庆石油学院,大庆163318 [2]北京大学,北京100871

出　　处：《生物技术通报》2007年第5期116-119,127,共5页Biotechnology Bulletin

摘　　要：脂肽是一类具有特殊作用的生物表面活性剂。本实验将血红蛋白基因(vhb)置于RDR细菌启动子驱动下的质粒PSET中,构建PSET-RDR-vhb重组质粒,并通过电激作用转入脂肽代谢菌株-枯草芽孢杆菌株(bacillus subtilis)ZW-3中,转化菌株经酶切和PCR电泳检测鉴定,Southern-blot杂交显示部分转化菌株中外源基因插入基因组DNA,采用一氧化碳差异色谱法测定了血红蛋白的表达量。实验进一步对转化菌株的生长曲线、总蛋白量、过氧化氢酶活性、脂肽的产率进行了测定,结果显示,相比于原始菌株,转化菌株数据均有明显提高。Hemoglobin of the gram-negative bacterium Vitreoscilla can bind oxygen strongly and reduce the oxygen requirement of the bacterium.A recombinant plasmid PEST-RDR-vhb was constructed and transferred into strain ZW-3（bacillus subtilis）,The results showed that the expression of vhb gene could improve cell growth and enhance the production of surfactin with 31.8% higher than that of control under different dissolved oxygen concentrations.Besides,vhb gene also increased the expression of total protein quantity and CAT.

关 键 词：透明颤菌血红蛋白基因 枯草芽孢杆菌 脂肽 表达 

分 类 号：Q78[生物学—分子生物学]